In vitro analysis of ISEcp1B-mediated mobilization of naturally occurring β-lactamase gene blaCTX-M of Kluyvera ascorbata

被引：138

作者：

Lartigue, MF ^{[1
]}

Poirel, L ^{[1
]}

Aubert, D ^{[1
]}

Nordmann, P ^{[1
]}

机构：

[1] Univ Paris 11, Fac Med Paris Sud, Assistance Publ Hop Paris, Hop Bicetre,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2006年 / 50卷 / 04期

关键词：

D O I：

10.1128/AAC.50.4.1282-1286.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

ISEcp1B has been reported to be associated with and to mobilize the emerging expanded-spectrum P-lactamase bla(CTX-M) genes in Enterobacteriaceae. Thus, the ability of this insertion sequence to mobilize the bla(CTX-M-2) gene was tested from its progenitor, Kluyvera ascorbata. Insertions of ISEcp1B upstream of the bla(CTX-M-2) gene in K. ascorbata reference strain CIP7953 were first selected with cefotaxime (0.5 and 2 mu g/ml). In those cases, ISEcp1B brought promoter sequences enhancing bla(CTX-M-2) expression in K. ascorbata. Then, ISEcp1B-mediated mobilization of the bla(CTX-M-2) gene from K. ascorbata to Escherichia coli J53 was attempted. The transposition frequency of ISEcp1B-bla(CTX-M-2) occurred at (6.4 +/- 0.5) x 10(-7) in E. coli. Cefotaxime, ceftazidime, and piperacillin enhanced transposition, whereas amoxicillin, cefuroxime, and nalidixic acid did not. Transposition was also enhanced when studied at 40 degrees C.

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页码：1282 / 1286

页数：5

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