PCR-amplified cDNA probes for verification of differentially expressed genes

被引：10

作者：

Ross, R

Kumpf, K

ReskeKunz, AB

机构：

[1] Johannes Gutenberg University, Mainz

[2] Clinical Research Unit, Department of Dermatology, Johannes Gutenberg-University, D-55131 Mainz

来源：

BIOTECHNIQUES | 1997年 / 22卷 / 05期

关键词：

D O I：

10.2144/97225st01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential display has proven to be a powerful technique for the detection and isolation of differentially expressed genes. By generating reproducible cDNA expression patterns, it is possible to compare gene expression by two or more cell types, developmental stages or tissues and to isolate as yet unknown differentially expressed genes. A sensitive method is necessary to verify the differential expression of the isolated cDNAs. Here we describe the use of adaptor-ligated, PCR-amplified total cDNA of the two cell types compared as a probe for Southern hybridizations with the isolated cDNAs.

引用

收藏

页码：894 / 897

页数：4

相关论文

共 12 条

[1]

BAUER D, 1994, PCR METH APPL, V4, P97

[2] BIOCHEMICAL-PROPERTIES OF MHC CLASS-II MOLECULES ENDOGENOUSLY SYNTHESIZED AND EXPRESSED BY MOUSE LANGERHANS CELLS [J].

BECKER, D ;

RESKEKUNZ, AB ;

KNOP, J ;

RESKE, K .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1991, 21 (05) :1213-1220

[3] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[4] A METHOD FOR THE RAPID ISOLATION OF HUMAN EPIDERMAL LANGERHANS CELLS USING IMMUNOMAGNETIC MICROSPHERES [J].

HANAU, D ;

SCHMITT, DA ;

FABRE, M ;

CAZENAVE, JP .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1988, 91 (03) :274-279

[5] GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR AND INTERLEUKIN-1 MEDIATE THE MATURATION OF MURINE EPIDERMAL LANGERHANS CELLS INTO POTENT IMMUNOSTIMULATORY DENDRITIC CELLS [J].

HEUFLER, C ;

KOCH, F ;

SCHULER, G .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (02) :700-705

[6] DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION [J].

LIANG, P ;

PARDEE, AB .

SCIENCE, 1992, 257 (5072) :967-971

[7] RECENT ADVANCES IN DIFFERENTIAL DISPLAY [J].

LIANG, P ;

PARDEE, AB .

CURRENT OPINION IN IMMUNOLOGY, 1995, 7 (02) :274-280

[8]

Maniatis T., 1982, MOL CLONING

[9] IMPROVEMENTS TO THE DIFFERENTIAL DISPLAY METHOD FOR GENE ANALYSIS [J].

MOU, LJ ;

MILLER, H ;

LI, J ;

WANG, E ;

CHALIFOUR, L .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 199 (02) :564-569

[10] NON-COORDINATE SYNTHESIS OF MHC CLASS-II PROTEINS AND INVARIANT CHAINS BY EPIDERMAL LANGERHANS CELLS DERIVED FROM SHORT-TERM IN-VITRO CULTURE [J].

NEISS, U ;

RESKE, K .

INTERNATIONAL IMMUNOLOGY, 1994, 6 (01) :61-71