Quantitative detection of bisphenol A and bisphenol A diglycidyl ether metabolites in human plasma by liquid chromatography-electrospray mass spectrometry

被引:62
作者
Inoue, K
Yamaguchi, A
Wada, M
Yoshimura, Y
Makino, T
Nakazawa, H
机构
[1] Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, Tokyo 1428501, Japan
[2] Tokai Univ, Sch Med, Dept Obstet & Gynecol, Isehara, Kanagawa 251193, Japan
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2001年 / 765卷 / 02期
关键词
bisphenol A; bisphenol A diglycidyl ether;
D O I
10.1016/S0378-4347(01)00393-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Due to the ubiquity of epoxy resin compounds and their potential role in increasing the risk for reproductive dysfunction and cancer, the need for an assessment of human exposure is urgent. Therefore, we developed a method for measuring bisphenol A (BPA) and bisphenol A diglycidyl ether (BADGE) metabolites in human blood samples using high-performance liquid chromatography-electrospray ionization mass spectrometry (LC-MS). Human blood samples were processed using enzymatic deconjugation of the glucuronides followed by a novel sample preparation procedure using a solid-phase-cartridge column. This selective analytical method permits rapid detection of the metabolites, free BPA and a hydrolysis product of BADGE (BADGE-4OH) with detection limits in the low nanogram per milliliter range (0.1 ng ml(-1) of BPA and 0.5 ng ml(-1) of BADGE-4OH). The sample extraction was achieved by Oasis HLB column on gradient elution. The recoveries of BPA and BADGE-4OH added to human plasma samples were above 70.0% with a standard deviation of less than 5.0%. This selective, sensitive and accurate method will assist in elucidating potential associations between human exposure to epoxy-based compounds and adverse health effects. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:121 / 126
页数:6
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