CRISPR-Cas immunity in prokaryotes

被引：604

作者：

Marraffini, Luciano A. ^{[1
]}

机构：

[1] Rockefeller Univ, Bacteriol Lab, New York, NY 10065 USA

来源：

NATURE | 2015年 / 526卷 / 7571期

关键词：

HORIZONTAL GENE-TRANSFER; SEQUENCE-SPECIFIC ANTIMICROBIALS; GUIDED SURVEILLANCE COMPLEX; ADAPTIVE BACTERIAL IMMUNITY; IN-VITRO RECONSTITUTION; R-LOOP FORMATION; ESCHERICHIA-COLI; STREPTOCOCCUS-THERMOPHILUS; SPACER ACQUISITION; CRYSTAL-STRUCTURE;

D O I：

10.1038/nature15386

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Prokaryotic organisms are threatened by a large array of viruses and have developed numerous defence strategies. Among these, only clustered, regularly interspaced short palindromic repeat (CRISPR)-Cas systems provide adaptive immunity against foreign elements. Upon viral injection, a small sequence of the viral genome, known as a spacer, is integrated into the CRISPR locus to immunize the host cell. Spacers are transcribed into small RNA guides that direct the cleavage of the viral DNA by Cas nucleases. Immunization through spacer acquisition enables a unique form of evolution whereby a population not only rapidly acquires resistance to its predators but also passes this resistance mechanism vertically to its progeny.

引用

页码：55 / 61

页数：7

共 120 条

[1] An Escherichia coli chromosomal ''addiction module'' regulated by 3',5'-bispyrophosphate: A model for programmed bacterial cell death [J].

Aizenman, E ;

EngelbergKulka, H ;

Glaser, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) :6059-6063

[2] Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease [J].

Anders, Carolin ;

Niewoehner, Ole ;

Duerst, Alessia ;

Jinek, Martin .

NATURE, 2014, 513 (7519) :569-+

[3] Virus population dynamics and acquired virus resistance in natural microbial communities [J].

Andersson, Anders F. ;

Banfield, Jillian F. .

SCIENCE, 2008, 320 (5879) :1047-1050

[4] Detection and characterization of spacer integration intermediates in type I-E CRISPR-Cas system [J].

Arslan, Zihni ;

Hermanns, Veronica ;

Wurm, Reinhild ;

Wagner, Rolf ;

Pul, Uemit .

NUCLEIC ACIDS RESEARCH, 2014, 42 (12) :7884-7893

[5] CRISPR provides acquired resistance against viruses in prokaryotes [J].

Barrangou, Rodolphe ;

Fremaux, Christophe ;

Deveau, Helene ;

Richards, Melissa ;

Boyaval, Patrick ;

Moineau, Sylvain ;

Romero, Dennis A. ;

Horvath, Philippe .

SCIENCE, 2007, 315 (5819) :1709-1712

[6] HIGH ABUNDANCE OF VIRUSES FOUND IN AQUATIC ENVIRONMENTS [J].

BERGH, O ;

BORSHEIM, KY ;

BRATBAK, G ;

HELDAL, M .

NATURE, 1989, 340 (6233) :467-468

[7] BIOLOGY OF DNA RESTRICTION [J].

BICKLE, TA ;

KRUGER, DH .

MICROBIOLOGICAL REVIEWS, 1993, 57 (02) :434-450

[8] Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials [J].

Bikard, David ;

Euler, Chad W. ;

Jiang, Wenyan ;

Nussenzweig, Philip M. ;

Goldberg, Gregory W. ;

Duportet, Xavier ;

Fischetti, Vincent A. ;

Marraffini, Luciano A. .

NATURE BIOTECHNOLOGY, 2014, 32 (11) :1146-1150

[9] CRISPR Interference Can Prevent Natural Transformation and Virulence Acquisition during In Vivo Bacterial Infection [J].

Bikard, David ;

Hatoum-Aslan, Asma ;

Mucida, Daniel ;

Marraffini, Luciano A. .

CELL HOST & MICROBE, 2012, 12 (02) :177-186

[10] The major head protein of bacteriophage T4 binds specifically to elongation factor Tu [J].

Bingham, R ;

Ekunwe, SIN ;

Falk, S ;

Snyder, L ;

Kleanthous, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (30) :23219-23226

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