Structure of the Yeast Mitochondrial Large Ribosomal Subunit

被引：454

作者：

Amunts, Alexey ^{[1
]}

Brown, Alan ^{[1
]}

Bai, Xiao-chen ^{[1
]}

Llacer, Jose L. ^{[1
]}

Hussain, Tanweer ^{[1
]}

Emsley, Paul ^{[1
]}

Long, Fei ^{[1
]}

Murshudov, Garib ^{[1
]}

Scheres, Sjors H. W. ^{[1
]}

Ramakrishnan, V. ^{[1
]}

机构：

[1] MRC Lab Mol Biol, Cambridge CB2 0QH, England

来源：

SCIENCE | 2014年 / 343卷 / 6178期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

EM STRUCTURE DETERMINATION; CRYO-EM; ANGSTROM RESOLUTION; CRYSTAL-STRUCTURE; EUKARYOTIC RIBOSOME; TRANSFER-RNA; TUNNEL EXIT; PROTEINS; EVOLUTION; PROTEOME;

D O I：

10.1126/science.1249410

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts. We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.

引用

页码：1485 / 1489

页数：5

共 38 条

[1] Structural aspects of mitochondrial translational apparatus [J].