The Structure of the Eukaryotic Ribosome at 3.0 Å Resolution

被引：859

作者：

Ben-Shem, Adam ^{[1
]}

de Loubresse, Nicolas Garreau

Melnikov, Sergey

Jenner, Lasse

Yusupova, Gulnara

Yusupov, Marat

机构：

[1] Inst Genet & Biol Mol & Cellulaire, F-67400 Illkirch Graffenstaden, France

来源：

SCIENCE | 2011年 / 334卷 / 6062期

关键词：

SACCHAROMYCES-CEREVISIAE; CRYSTAL-STRUCTURE; TRANSLATION INITIATION; 80S RIBOSOME; PROTEIN-SYNTHESIS; RNA; SUBUNIT; IRES; REINITIATION; MECHANISM;

D O I：

10.1126/science.1212642

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ribosomes translate genetic information encoded by messenger RNA into proteins. Many aspects of translation and its regulation are specific to eukaryotes, whose ribosomes are much larger and intricate than their bacterial counterparts. We report the crystal structure of the 80S ribosome from the yeast Saccharomyces cerevisiae-including nearly all ribosomal RNA bases and protein side chains as well as an additional protein, Stm1-at a resolution of 3.0 angstroms. This atomic model reveals the architecture of eukaryote-specific elements and their interaction with the universally conserved core, and describes all eukaryote-specific bridges between the two ribosomal subunits. It forms the structural framework for the design and analysis of experiments that explore the eukaryotic translation apparatus and the evolutionary forces that shaped it.

引用

页码：1524 / 1529

页数：6

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