Endocytosis, intracellular transport, and exocytosis of lanthanide-doped upconverting nanoparticles in single living cells

被引：141

作者：

Bae, Yun Mi ^{[1
,2
]}

Park, Yong Il ^{[3
]}

Nam, Sang Hwan ^{[1
]}

Kim, Jeong Hyun ^{[3
]}

Lee, Kyunghee ^{[1
]}

Kim, Hyung Min ^{[1
]}

Yoo, Byeongjun ^{[3
]}

Choi, Joon Sig ^{[2
]}

Lee, Kang Taek ^{[1
]}

Hyeon, Taeghwan ^{[3
]}

Suh, Yung Doug ^{[1
]}

机构：

[1] Korea Res Inst Chem Technol, Res Ctr Convergence Nanotechnol, Lab Adv Mol Probing LAMP, Taejon 305600, South Korea

[2] Chungnam Natl Univ, Dept Biochem, Taejon 305764, South Korea

[3] Seoul Natl Univ, Sch Chem & Biol Engn, World Class Univ WCU, Program Chem Convergence Energy & Environm C2E2, Seoul 151744, South Korea

来源：

BIOMATERIALS | 2012年 / 33卷 / 35期

关键词：

Nanoparticle; Image analysis; Fluorescence; Molecular imaging; Cytotoxicity; QUANTUM DOTS; CELLULAR UPTAKE; UP-CONVERSION; TRACKING; INSIGHTS; IONS;

D O I：

10.1016/j.biomaterials.2012.08.039

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Lanthanide-doped upconverting nanoparticles (UCNPs) have recently attracted enormous attention in the field of biological imaging owing to their unique optical properties (near-infrared excitation followed by photoluminescence in the visible spectral range). For biological applications, it is critical to understand the interaction between these nanoparticles and biological systems at the cellular level. In this paper, using epi-fluorescence microscopy with 980-nm excitation, a full intracellular pathway composed of endocytosis, active transport, and exocytosis was clearly visualized for PEG-phospholipid-coated UCNPs in single HeLa cells, which was experimentally feasible mostly thanks to the excellent photostability and low cytotoxicity thereof. Each step in the pathway was characterized and identified by various chemical inhibition studies and spectroscopic measurements. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：9080 / 9086

页数：7

共 31 条

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