Tracking individual kinesin motors in living cells using single quantum-dot imaging

被引：251

作者：

Courty, Sebastien

Luccardini, Camilla

Bellaiche, Yohanns

Cappello, Giovanni

Dahan, Maxime

机构：

[1] Inst Curie, CNRS, UMR 168, F-75005 Paris, France

[2] Inst Curie, CNRS, UMR 144, F-75005 Paris, France

[3] Univ Paris 06, Ecole Normale Super, CNRS UMR 8552, Lab Kastler Brossel, F-75005 Paris, France

来源：

NANO LETTERS | 2006年 / 6卷 / 07期

关键词：

D O I：

10.1021/nl060921t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a simple method using semiconductor quantum dots (QDs) to track the motion of intracellular proteins with a high sensitivity. We characterized the in vivo motion of individual QD-tagged kinesin motors in living HeLa cells. Single-molecule measurements provided important parameters of the motor, such as its velocity and processivity, as well as an estimate of the force necessary to carry a QD. Our measurements demonstrate the importance of single-molecule experiments in the investigation of intracellular transport as well as the potential of single quantum-dot imaging for the study of important processes such as cellular trafficking, cell polarization, and division.

引用

页码：1491 / 1495

页数：5

共 31 条

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