MIFlowCyt: The Minimum Information about a Flow Cytometry Experiment

被引：365

作者：

Lee, Jamie A. ^{[2
]}

Spidlen, Josef ^{[1
]}

Boyce, Keith ^{[3
]}

Cai, Jennifer ^{[2
]}

Crosbie, Nicholas ^{[4
]}

Dalphin, Mark ^{[5
]}

Furlong, Jeff ^{[6
]}

Gasparetto, Maura. ^{[1
]}

Goldberg, Michael ^{[7
]}

Goralczyk, Elizabeth M. ^{[8
]}

Hyun, Bill ^{[9
]}

Jansen, Kirstin

Kollmann, Tobias ^{[10
]}

Kong, Megan ^{[2
]}

Leif, Robert ^{[11
]}

McWeeney, Shannon ^{[12
,13
,14
]}

Moloshok, Thomas D. ^{[8
]}

Moore, Wayne ^{[15
]}

Nolan, Garry ^{[16
]}

Nolan, John ^{[17
]}

Nikolich-Zugich, Janko ^{[18
,19
]}

Parrish, David ^{[3
]}

Purcell, Barclay ^{[20
]}

Qian, Yu ^{[2
]}

Selvaraj, Biruntha ^{[20
]}

Smith, Clayton ^{[1
]}

Tchuvatkina, Olga ^{[7
]}

Wertheimer, Anne ^{[19
]}

WilkinSon, Peter ^{[21
]}

Wilson, Christopher ^{[6
]}

Wood, James ^{[22
]}

Zigon, Robert ^{[23
]}

Scheuermann, Richard H. ^{[2
,24
]}

Brinkman, Ryan R. ^{[1
]}

机构：

[1] British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada

[2] Univ Texas SW Med Ctr Dallas, Dept Pathol, Dallas, TX 75390 USA

[3] Immune Tolerance Network, Pittsburgh, PA 15238 USA

[4] Inivai Technol, Mentone, Vic 3194, Australia

[5] Amgen Inc, Thousand Oaks, CA 91320 USA

[6] Univ Washington, Dept Immunol, Seattle, WA 98195 USA

[7] BD Biosci, San Jose, CA 95131 USA

[8] Fox Chase Canc Ctr, Philadelphia, PA 19111 USA

[9] Univ Calif San Francisco, Lab Cell Anal, San Francisco, CA 94143 USA

[10] Univ British Columbia, Vaccine Evaluat Ctr, Child & Family Res Inst, Dept Pediat, Vancouver, BC V5Z 4H4, Canada

[11] Newport Instruments, San Diego, CA 92115 USA

[12] Oregon Hlth & Sci Univ, OHSU Canc Inst, Portland, OR 97006 USA

[13] Oregon Hlth & Sci Univ, Dept Publ Hlth & Prevent Med, Div Biostat, Portland, OR 97006 USA

[14] Oregon Hlth & Sci Univ, Oregon Clin & Translat Res Inst, Portland, OR 97006 USA

[15] Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA

[16] Stanford Univ, Dept Microbiol & Immunol, Stanford, CA 94305 USA

[17] La Jolla Bioengn Inst, La Jolla, CA 92037 USA

[18] Oregon Hlth & Sci Univ, Dept Mol Microbiol & Immunol, Vaccine & Gene Therapy Inst, Portland, OR 97006 USA

[19] Oregon Hlth & Sci Univ, Oregon Natl Primate Res Ctr, Portland, OR 97006 USA

[20] Cytopeia Inc, Seattle, WA 98125 USA

[21] Univ Montreal, Montreal, PQ H3C 3J7, Canada

[22] Wake Forest Univ, Bowman Gray Sch Med, Dept Canc Biol, Winston Salem, NC 27157 USA

[23] Beckman Coulter Inc, Indianapolis, IN USA

[24] Univ Texas SW Med Ctr Dallas, Div Biomed Informat, Dallas, TX 75390 USA

来源：

CYTOMETRY PART A | 2008年 / 73A卷 / 10期

关键词：

immunology; fluorescence-activated cell sorting; knowledge representation;

D O I：

10.1002/cyto.a.20623

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fundamental tenet of scientific research is that Published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental;I findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross-disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the experiment, the configuration of the instrument used to perforin the assays, and the data processing approaches used to interpret the primary Output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoption of MIFlowCYT by the scientific and publishing communities will facilitate third-party Understanding and reuse of FCM data. (C) 2008 International Society for Advancement Cytometry.

引用

页码：926 / 930

页数：5

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