Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae

被引：568

作者：

Pelkmans, L ^{[1
]}

Püntener, D ^{[1
]}

Helenius, A ^{[1
]}

机构：

[1] ETH Honggerberg, Swiss Fed Inst Technol ETHZ, CH-8093 Zurich, Switzerland

来源：

SCIENCE | 2002年 / 296卷 / 5567期

关键词：

D O I：

10.1126/science.1069784

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Simian virus 40 (SV40) utilizes endocytosis through caveolae for infectious entry into host cells. We found that after binding to caveolae, virus particles induced transient breakdown of actin stress fibers. Actin was then recruited to virus-loaded caveolae as actin patches that served as sites for actin "tail" formation. Dynamin II was also transiently recruited. These events depended on the presence of cholesterol and on the activation of tyrosine kinases that phosphorylated proteins in caveolae. They were necessary for formation of caveolae-derived endocytic vesicles and for infection of the cell. Thus, caveolar endocytosis is ligand-triggered and involves extensive rearrangement of the actin cytoskeleton.

引用

页码：535 / 539

页数：5

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