D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling

被引：663

作者：

Zhou, Feng ^{[1
,2
]}

Lin, Qibing ^{[2
]}

Zhu, Lihong ^{[1
]}

Ren, Yulong ^{[2
]}

Zhou, Kunneng ^{[1
]}

Shabek, Nitzan ^{[3
,4
]}

Wu, Fuqing ^{[2
]}

Mao, Haibin ^{[3
,4
]}

Dong, Wei ^{[2
]}

Gan, Lu ^{[2
]}

Ma, Weiwei ^{[2
]}

Gao, He ^{[1
]}

Chen, Jun ^{[2
]}

Yang, Chao ^{[1
]}

Wang, Dan ^{[2
]}

Tan, Junjie ^{[2
]}

Zhang, Xin ^{[2
]}

Guo, Xiuping ^{[2
]}

Wang, Jiulin ^{[2
]}

Jiang, Ling ^{[1
]}

Liu, Xi ^{[1
]}

Chen, Weiqi ^{[5
]}

Chu, Jinfang ^{[5
]}

Yan, Cunyu ^{[5
]}

Ueno, Kotomi ^{[6
]}

Ito, Shinsaku ^{[6
]}

Asami, Tadao ^{[6
]}

Cheng, Zhijun ^{[2
]}

Wang, Jie ^{[2
]}

Lei, Cailin ^{[2
]}

Zhai, Huqu ^{[2
]}

Wu, Chuanyin ^{[2
]}

Wang, Haiyang ^{[2
]}

Zheng, Ning ^{[3
,4
]}

Wan, Jianmin ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

[2] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

[3] Univ Washington, Dept Pharmacol, Seattle, WA 98195 USA

[4] Univ Washington, Howard Hughes Med Inst, Seattle, WA 98195 USA

[5] Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China

[6] Univ Tokyo, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan

来源：

NATURE | 2013年 / 504卷 / 7480期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

BOX PROTEIN TIR1; GENE-EXPRESSION; ARABIDOPSIS; RICE; PERCEPTION; OUTGROWTH; MAX2; GERMINATION; INHIBITION; KARRIKIN;

D O I：

10.1038/nature12878

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Strigolactones (SLs), a newly discovered class of carotenoid-derived phytohormones, are essential for developmental processes that shape plant architecture and interactions with parasitic weeds and symbiotic arbuscular mycorrhizal fungi. Despite the rapid progress in elucidating the SL biosynthetic pathway, the perception and signalling mechanisms of SL remain poorly understood. Here we show that DWARF 53 (D53) acts as a repressor of SL signalling and that SLs induce its degradation. We find that the rice (Oryza sativa) d53 mutant, which produces an exaggerated number of tillers compared to wild-type plants, is caused by a gain-of-function mutation and is insensitive to exogenous SL treatment. The D53 gene product shares predicted features with the class I Clp ATPase proteins and can form a complex with the a/b hydrolase protein DWARF 14 (D14) and the F-box protein DWARF 3 (D3), two previously identified signalling components potentially responsible for SL perception. We demonstrate that, in a D14- and D3-dependent manner, SLs induce D53 degradation by the proteasome and abrogate its activity in promoting axillary bud outgrowth. Our combined genetic and biochemical data reveal that D53 acts as a repressor of the SL signalling pathway, whose hormone-induced degradation represents a key molecular link between SL perception and responses.

引用

页码：406 / +

页数：16

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[1] Arabidopsis BRANCHED1 acts as an integrator of branching signals within axillary buds [J].