Transgenic Citrus Expressing an Arabidopsis NPR1 Gene Exhibit Enhanced Resistance against Huanglongbing (HLB; Citrus Greening)

被引：136

作者：

Dutt, Manjul ^{[1
]}

Barthe, Gary ^{[1
]}

Irey, Michael ^{[2
]}

Grosser, Jude ^{[1
]}

机构：

[1] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA

[2] Southern Gardens Citrus, Clewiston, FL USA

来源：

PLOS ONE | 2015年 / 10卷 / 09期

关键词：

CANDIDATUS LIBERIBACTER ASIATICUS; WRKY70 TRANSCRIPTION FACTOR; SALICYLIC-ACID INDUCTION; REAL-TIME PCR; DIAPHORINA-CITRI; DEFENSE RESPONSE; SWEET ORANGE; DISEASE; PROTEIN; OVEREXPRESSION;

D O I：

10.1371/journal.pone.0137134

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Commercial sweet orange cultivars lack resistance to Huanglongbing (HLB), a serious phloem limited bacterial disease that is usually fatal. In order to develop sustained disease resistance to HLB, transgenic sweet orange cultivars 'Hamlin' and 'Valencia' expressing an Arabidopsis thaliana NPR1 gene under the control of a constitutive CaMV 35S promoter or a phloem specific Arabidopsis SUC2 (AtSUC2) promoter were produced. Overexpression of AtNPR1 resulted in trees with normal phenotypes that exhibited enhanced resistance to HLB. Phloem specific expression of NPR1 was equally effective for enhancing disease resistance. Transgenic trees exhibited reduced diseased severity and a few lines remained disease-free even after 36 months of planting in a high-disease pressure field site. Expression of the NPR1 gene induced expression of several native genes involved in the plant defense signaling pathways. The AtNPR1 gene being plant derived can serve as a component for the development of an all plant T-DNA derived consumer friendly GM tree.

引用

页数：17

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