Observation of conserved solution-phase secondary structure in gas-phase tryptic peptides

被引：50

作者：

Ruotolo, BT

Verbeck, GF

Thomson, LM

Gillig, KJ

Russell, DH ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, Lab Biol Mass Spectrometry, College Stn, TX 77843 USA

[2] Texas A&M Univ, Lab Mol Simulat, College Stn, TX 77843 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 16期

关键词：

D O I：

10.1021/ja0178113

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Results from ion mobility studies of tryptic peptides suggest that, in some cases, the gas-phase structures can be related to the solution-phase structure of the parent protein. The interpretation of ion mobility measurements is supported by results from molecular modeling and H/D exchange experiments on the same peptides. This study clearly illustrates the utility of IM-MS for screening complex mixtures for peptides having intrinsically stable secondary/tertiary structures, and/or posttranslational modification. Copyright © 2002 American Chemical Society.

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页码：4214 / 4215

页数：2

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