Coupling high-pressure MALDI with ion mobility/orthogonal time-of flight mass spectrometry

被引:132
作者
Gillig, KJ
Ruotolo, B
Stone, EG
Russell, DH [1 ]
Fuhrer, K
Gonin, M
Schultz, AJ
机构
[1] Texas A&M Univ, Dept Chem, Lab Biol Mass Spectrometry, College Stn, TX 77843 USA
[2] Ionwerks Inc, Houston, TX 77005 USA
关键词
D O I
10.1021/ac0005619
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new ion mobility/time-of-flight mass spectrometer employing a high-pressure MALDI source has been designed and tested, The prototype instrument operates at a source/drift cell pressure of 1-10 Torr helium, resulting in a mobility resolution of similar to 25. A small time-of-flight mass spectrometer (20 cm) with a mass resolution of up to 200 has been attached to the drift cell to identify (in terms of mass-to-charge ratio) the separated ions. A simple tripeptide mixture has been separated in the drift tube and mass identified as singly protonated species. The ability to separate peptide mixtures, e.g., tryptic digest of a protein, is illustrated and compared to results obtained on a high vacuum time-of-flight instrument.
引用
收藏
页码:3965 / 3971
页数:7
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