Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue

被引：121

作者：

Ingaramo, Maria ^{[1
]}

York, Andrew G. ^{[1
]}

Wawrzusin, Peter ^{[1
]}

Milberg, Oleg ^{[2
]}

Hong, Amy ^{[3
]}

Weigert, Roberto ^{[2
]}

Shroff, Hari ^{[1
]}

Patterson, George H. ^{[1
]}

机构：

[1] Natl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD 20892 USA

[2] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20892 USA

[3] NHLBI, NIH, Bethesda, MD 20892 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 14期

基金：

美国国家卫生研究院;

关键词：

multiphoton; superresolution; DIFFRACTION-LIMIT; RESOLUTION LIMIT; FLUORESCENCE; PINHOLE; ARRAY; LIVE; BREAKING; SINGLE;

D O I：

10.1073/pnas.1314447111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 mu m, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient two-photon excitation. Two-photon MSIM gives resolution-doubled images with better sectioning and contrast in thick scattering samples such as Caenorhabditis elegans embryos, Drosophila melanogaster larval salivary glands, and mouse liver tissue.

引用

页码：5254 / 5259

页数：6

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