Destabilization of quadruplex DNA by 8-aminoguanine

被引：16

作者：

de la Osa, JP

González, C

Gargallo, R

Rueda, M

Cubero, E

Orozco, M

Aviñó, A

Eritja, R

机构：

[1] CSIC, Inst Quim Fis Rocasolano, E-28006 Madrid, Spain

[2] Univ Barcelona, Fac Quim, Dept Quim Analit, E-08028 Barcelona, Spain

[3] Univ Barcelona, Fac Quim, Dept Bioquim & Biol Mol, E-08028 Barcelona, Spain

[4] IRBB PCB, Mol Modeling Unit, E-08028 Barcelona, Spain

[5] Barcelona Supercomp Ctr, Life sci Program, ES-08034 Barcelona, Spain

[6] CSIC, Inst Biol Mol Barcelona, ES-08034 Barcelona, Spain

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 01期

关键词：

DNA; NMR spectroscopy; oligonucleotides; quadruplexes; triplexes;

D O I：

10.1002/cbic.200500281

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Getting rid of unwanted quadruplexes Oligonucleotides carrying 8-amino-2′-deoxyguanosine form very stable triple-helical structures. In this paper, we show that 8-amino-2′-deoxyguanosines destabilize quadruplex DNA structures. This quadruplex-destabilization effect is very convenient for the design of triplex-forming oligonucleotides, making 8-aminopurines superior to other purine derivatives. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：46 / 48

页数：3

相关论文

共 32 条

[1] Synthesis and properties of triple helix-forming oligodeoxyribonucleotides containing 7-chloro-7-deaza-2′-deoxyguanosine [J].

Aubert, Y ;

Perrouault, L ;

Hélène, C ;

Giovannangeli, C ;

Asseline, U .

BIOORGANIC & MEDICINAL CHEMISTRY, 2001, 9 (06) :1617-1624

[2] Properties of triple helices formed by parallel-stranded hairpins containing 8-aminopurines [J].

Aviñó, A ;

Frieden, M ;

Morales, JC ;

de la Torre, BG ;

García, RG ;

Azorín, F ;

Gelpí, JL ;

Orozco, M ;

González, C ;

Eritja, R .

NUCLEIC ACIDS RESEARCH, 2002, 30 (12) :2609-2619

[3] Parallel-stranded hairpins containing 8-aminopurines.: Novel efficient probes for triple-helix formation [J].

Aviñó, A ;

Morales, JC ;

Frieden, M ;

de la Torre, BG ;

García, RG ;

Cubero, E ;

Luque, FJ ;

Orozco, M ;

Azorín, F ;

Eritja, R .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2001, 11 (13) :1761-1763

[4] Triplex DNA: Fundamentals, advances, and potential applications for gene therapy [J].

Chan, PP ;

Glazer, PM .

JOURNAL OF MOLECULAR MEDICINE-JMM, 1997, 75 (04) :267-282

[5] Self-association of G-rich oligodeoxyribonucleotides under conditions promoting purine-motif triplex formation [J].

Cheng, AJ ;

Wang, JC ;

Van Dyke, MW .

ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 1998, 8 (03) :215-225

[6] Hoogsteen-based parallel-stranded duplexes of DNA.: Effect of 8-amino-purine derivatives [J].

Cubero, E ;

Aviñó, A ;

de la Torre, BG ;

Frieden, M ;

Eritja, R ;

Luque, FJ ;

González, C ;

Orozco, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (12) :3133-3142

[7] Biophysical and biological properties of quadruplex oligodeoxyribonucleotides [J].

Dapic, V ;

Abdomerovic, V ;

Marrington, R ;

Peberdy, J ;

Rodger, A ;

Trent, JO ;

Bates, PJ .

NUCLEIC ACIDS RESEARCH, 2003, 31 (08) :2097-2107

[8] Triple helix stabilization properties of oligonucleotides containing 8-amino-2′-deoxyguanosine [J].

García, RG ;

Bachi, A ;

Eritja, R ;

Luque, FJ ;

Orozco, M .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1998, 8 (21) :3011-3016

[9] TRIPLER FORMATION AT THE RAT NEU GENE UTILIZING IMIDAZOLE AND 2'-DEOXY-6-THIOGUANOSINE BASE SUBSTITUTIONS [J].

GEE, JE ;

REVANKAR, GR ;

RAO, TS ;

HOGAN, ME .

BIOCHEMISTRY, 1995, 34 (06) :2042-2048

[10] Synthesis and triplex-forming properties of cyclic oligonucleotides with (G,A)-antiparallel strands [J].

Grimau, MG ;

Aviñó, A ;

Gargallo, R ;

Eritja, R .

CHEMISTRY & BIODIVERSITY, 2005, 2 (02) :275-285

← 1 2 3 4 →