Long 5′ leaders inhibit removal of a 3′ trailer from a precursor tRNA by mammalian tRMA 3′ processing endoribonuclease

被引：33

作者：

Nashimoto, M ^{[1
]}

Wesemann, DR ^{[1
]}

Geary, S ^{[1
]}

Tamura, M ^{[1
]}

Kaspar, RL ^{[1
]}

机构：

[1] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 13期

关键词：

D O I：

10.1093/nar/27.13.2770

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian tRNA 3' processing endoribonuclease (3' tRNase) can remove a 3' trailer from various pre-tRNAs without 5' leader nucleotides. To examine how 5' leader sequences affect 3' processing efficiency, we performed in vitro 3' processing reactions with purified pig 3' tRNase and pre-tRN(Arg)s containing a 13-nt 3' trailer and a 5' leader of various lengths. The 3' processing was slightly stimulated by 5' leaders containing up to 7 nt, whereas leaders of 9 nt or longer severely inhibited the reaction. Structure probing indicated that the 5' leader sequences had little effect on pre-tRNA folding, Similar results were obtained using pre-tRNA(Val)s containing a 5' leader of various lengths, We also investigated whether 3' tRNase can remove 3' trailers that are stably base-paired with 5' leaders to form an extended acceptor stem, Even such small 5' leaders as 3 and 6 nt, when base-paired with a 3' trailer, severely hindered removal of the 3' trailer by 3' tRNase.

引用

页码：2770 / 2776

页数：7

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