MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity

被引：125

作者：

Chu, Daniel K. W. ^{[1
]}

Hui, Kenrie P. Y. ^{[1
]}

Perera, Ranawaka A. P. M. ^{[1
]}

Miguel, Eve ^{[2
,3
]}

Niemeyer, Daniela ^{[4
]}

Zhao, Jincun ^{[5
,6
]}

Channappanavar, Rudragouda ^{[5
]}

Dudas, Gytis ^{[7
]}

Oladipo, Jamiu O. ^{[1
,8
]}

Traore, Amadou ^{[9
]}

Fassi-Fihri, Ouafaa ^{[10
]}

Ali, Abraham ^{[11
]}

Demissie, Getnet F. ^{[12
]}

Muth, Doreen ^{[4
]}

Chan, Michael C. W. ^{[1
]}

Nicholls, John M. ^{[13
]}

Meyerholz, David K. ^{[14
]}

Kuranga, Sulyman A.

Mamo, Gezahegne ^{[15
]}

Zhou, Ziqi ^{[1
]}

So, Ray T. Y. ^{[1
]}

Hemida, Maged G. ^{[16
,17
]}

Webby, Richard J. ^{[18
]}

Roger, Francois ^{[2
,19
]}

Rambaut, Andrew ^{[20
,21
]}

Poon, Leo L. M. ^{[1
]}

Perlman, Stanley ^{[5
]}

Drosten, Christian ^{[4
]}

Chevalier, Veronique ^{[2
,22
]}

Peiris, Malik ^{[1
]}

机构：

[1] Univ Hong Kong, Li Ka Shing Fac Med, Sch Publ Hlth, Hong Kong, Hong Kong, Peoples R China

[2] Univ Montpellier, INRA, Ctr Cooperat Int Rech Agron Dev, Anim Sante Territoires Risques & Ecosyst, F-34398 Montpellier, France

[3] Univ Montpellier, CNRS, Maladies Infectieuses & Vecteurs Ecol Genet Evolu, Inst Rech Dev, F-34398 Montpellier, France

[4] Charite, Inst Virol, Campus Charite Mitte, D-10117 Berlin, Germany

[5] Univ Iowa, Dept Microbiol & Immunol, Iowa City, IA 52242 USA

[6] Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Dis, Natl Clin Res Ctr Resp Dis,State Key Lab Resp Dis, Guangzhou 510000, Guangdong, Peoples R China

[7] Fred Hutchinson Canc Res Ctr, Vaccine & Infect Dis Div, Seattle, WA 98109 USA

[8] Univ Ilorin, Fac Clin Sci, Dept Surg, Ilorin, Nigeria

[9] Ctr Natl Rech Sci & Technol, Inst lEnvironnem & Rechs Agricoles Burkina Faso, Lab Biol & Sante Anim, 04 BP 8645, Ouagadougou O4, Burkina Faso

[10] Hassan II Univ, Inst Agron & Vet, Rabat Inst, BP 6202, Rabat, Morocco

[11] Ethiopian Publ Hlth Inst, Bacterial Parasit & Zoonot Dis Res Directorate, Addis Ababa, Ethiopia

[12] Haramaya Univ, Coll Vet Med, Dire Dawa, Ethiopia

[13] Univ Hong Kong, Li Ka Shing Fac Med, Dept Pathol, Hong Kong, Hong Kong, Peoples R China

[14] Univ Iowa, Dept Pathol, Iowa City, IA 52242 USA

[15] Addis Ababa Univ, Coll Vet Med & Agr, Dept Vet Microbiol Immunol & Publ Hlth, Addis Ababa, Ethiopia

[16] King Faisal Univ, Coll Vet Med, Dept Microbiol & Parasitol, Al Hasa, Saudi Arabia

[17] Kafrelsheikh Univ, Dept Virol, Fac Vet Med, Kafrelsheikh, Egypt

[18] St Jude Childrens Res Hosp, Dept Infect Dis, Memphis, TN 38105 USA

[19] Kasetsart Univ, Bangkok 10900, Thailand

[20] Univ Edinburgh, Inst Evolutionary Biol, Edinburgh EH9 2FL, Midlothian, Scotland

[21] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA

[22] Inst Pasteur Cambodge, Phnom Penh, Cambodia

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2018年 / 115卷 / 12期

基金：

英国惠康基金;

关键词：

MERS; coronavirus; evolution; Africa; zoonosis; RESPIRATORY SYNDROME CORONAVIRUS; DROMEDARY CAMELS; INFECTION; TROPISM; PROTEIN; BAT;

D O I：

10.1073/pnas.1718769115

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Middle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection. Although MERS-CoV infection is ubiquitous in dromedaries across Africa as well as in the Arabian Peninsula, zoonotic disease appears confined to the Arabian Peninsula. MERS-CoVs from Africa have hitherto been poorly studied. We genetically and phenotypically characterized MERS-CoV from dromedaries sampled in Morocco, Burkina Faso, Nigeria, and Ethiopia. Viruses from Africa (clade C) are phylogenetically distinct from contemporary viruses from the Arabian Peninsula (clades A and B) but remain antigenically similar in microneutralization tests. Viruses from West (Nigeria, Burkina Faso) and North (Morocco) Africa form a subclade, C1, that shares clade-defining genetic signatures including deletions in the accessory gene ORF4b. Compared with human and camel MERS-CoV from Saudi Arabia, virus isolates from Burkina Faso (BF785) and Nigeria (Nig1657) had lower virus replication competence in Calu-3 cells and in ex vivo cultures of human bronchus and lung. BF785 replicated to lower titer in lungs of human DPP4-transduced mice. A reverse genetics-derived recombinant MERS-CoV (EMC) lacking ORF4b elicited higher type I and III IFN responses than the isogenic EMC virus in Calu-3 cells. However, ORF4b deletions may not be the major determinant of the reduced replication competence of BF785 and Nig1657. Genetic and phenotypic differences in West African viruses may be relevant to zoonotic potential. There is an urgent need for studies of MERS-CoV at the animal-human interface.

引用

页码：3144 / 3149

页数：6

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