An orthopoxvirus-based vaccine reduces virus excretion after MERS-CoV infection in dromedary camels

被引：219

作者：

Haagmans, Bart L. ^{[1
]}

van den Brand, Judith M. A. ^{[1
]}

Raj, V. Stalin ^{[1
]}

Volz, Asisa ^{[2
]}

Wohlsein, Peter ^{[3
]}

Smits, Saskia L. ^{[1
]}

Schipper, Debby ^{[1
]}

Bestebroer, Theo M. ^{[1
]}

Okba, Nisreen ^{[1
]}

Fux, Robert ^{[2
]}

Bensaid, Albert ^{[4
]}

Foz, David Solanes ^{[4
]}

Kuiken, Thijs ^{[1
]}

Baumgaertner, Wolfgang ^{[3
]}

Segales, Joaquim ^{[4
,5
]}

Sutter, Gerd ^{[2
]}

Osterhaus, Albert D. M. E. ^{[1
,6
,7
]}

机构：

[1] Erasmus MC, Dept Virosci, Rotterdam, Netherlands

[2] Univ Munich, Inst Infect Dis & Zoonoses, German Ctr Infect Res DZIF, Munich, Germany

[3] Univ Vet Med, Dept Pathol, Hannover, Germany

[4] Univ Autonoma Barcelona, IRTA, CReSA, Bellaterra 08193, Spain

[5] Univ Autonoma Barcelona, Fac Vet, Dept Sanitat & Anat Anim, Bellaterra 08193, Spain

[6] Artemis One Hlth, Utrecht, Netherlands

[7] Univ Vet Med, Res Ctr Emerging Infect & Zoonoses RIZ, Hannover, Germany

来源：

SCIENCE | 2016年 / 351卷 / 6268期

关键词：

RESPIRATORY SYNDROME CORONAVIRUS; RECEPTOR-BINDING DOMAIN; SAUDI-ARABIA; SPIKE PROTEIN; MOUSE MODEL; ANTIBODIES; TRACT; REPLICATION; EXPOSURE; EFFICACY;

D O I：

10.1126/science.aad1283

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Middle East respiratory syndrome coronavirus (MERS-CoV) infections have led to an ongoing outbreak in humans, which was fueled by multiple zoonotic MERS-CoV introductions from dromedary camels. In addition to the implementation of hygiene measures to limit further camel-to-human and human-to-human transmissions, vaccine-mediated reduction of MERS-CoV spread from the animal reservoir may be envisaged. Here we show that a modified vaccinia virus Ankara (MVA) vaccine expressing the MERS-CoV spike protein confers mucosal immunity in dromedary camels. Compared with results for control animals, we observed a significant reduction of excreted infectious virus and viral RNA transcripts in vaccinated animals upon MERS-CoV challenge. Protection correlated with the presence of serum neutralizing antibodies to MERS-CoV. Induction of MVA-specific antibodies that cross-neutralize camelpox virus would also provide protection against camelpox.

引用

页码：77 / 81

页数：5

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