Barley (Hordeum vulgare) oxalate oxidase is a manganese-containing enzyme

被引：119

作者：

Requena, L ^{[1
]}

Bornemann, S ^{[1
]}

机构：

[1] John Innes Ctr Plant Sci Res, Dept Biol Chem, Norwich NR4 7UH, Norfolk, England

来源：

BIOCHEMICAL JOURNAL | 1999年 / 343卷

关键词：

EPR; germin; homology model; hydrogen peroxide;

D O I：

10.1042/0264-6021:3430185

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxalate oxidase (EC 1.2.3.4) catalyses the conversion of oxalate and dioxygen into CO2 and H2O2. The barley (Hordeum vulgare) seedling root enzyme was purified to homogeneity and shown by metal analysis and EPR spectroscopy to contain Mn(II) at up to 0.80 atom per subunit. The involvement of Mn and neither flavin, Cu nor Fe in the direct conversion of dioxygen to H2O2 makes oxalate oxidase unique. A model of the active site of the holoenzyme based on a homology model of the apoenzyme is proposed.

引用

页码：185 / 190

页数：6

共 35 条

[21] OXALATE, GERMIN, AND THE EXTRACELLULAR-MATRIX OF HIGHER-PLANTS
LANE, BG
[J]. FASEB JOURNAL, 1994, 8 (03) : 294 - 301
[22] STRUCTURE OF PHASEOLIN AT 2-CENTER-DOT-2 ANGSTROM RESOLUTION - IMPLICATIONS FOR A COMMON VICILIN/LEGUMIN STRUCTURE AND THE GENETIC-ENGINEERING OF SEED STORAGE PROTEINS
LAWRENCE, MC
IZARD, T
BEUCHAT, M
BLAGROVE, RJ
COLMAN, PM
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1994, 238 (05) : 748 - 776
[23] MANGANESE SEED CONTENT AND TAKE-ALL OF CEREALS
MCCAYBUIS, TS
HUBER, DM
GRAHAM, RD
PHILLIPS, JD
MISKIN, KE
[J]. JOURNAL OF PLANT NUTRITION, 1995, 18 (08) : 1711 - 1721
[24] NARROD SA, 1966, METHOD ENZYMOL, V8, P354
[25] PURIFICATION AND PROPERTIES OF OXALATE OXIDASE FROM SORGHUM LEAVES
PUNDIR, CS
[J]. PHYTOCHEMISTRY, 1991, 30 (04) : 1065 - 1067
[26] PUNDIR CS, 1993, BIOCHIM BIOPHYS ACTA, V1161, P1
[27] TIME-COURSE OF BIOSYNTHESIS OF PHENOLICS AND LIGNIN IN ROOTS OF WHEAT GENOTYPES DIFFERING IN MANGANESE EFFICIENCY AND RESISTANCE TO TAKE-ALL FUNGUS
RENGEL, Z
GRAHAM, RD
PEDLER, JF
[J]. ANNALS OF BOTANY, 1994, 74 (05) : 471 - 477
[28] Rost B, 1996, METHOD ENZYMOL, V266, P525
[29] SHIMADA M, 1994, FEMS MICROBIOL REV, V13, P285, DOI 10.1111/j.1574-6976.1994.tb00049.x
[30] SHIMAO M, 1983, FEMS MICROBIOL LETT, V20, P429

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