Cloning and sequence determination of a gene encoding an osmotin-like protein from strawberry (Fragaria x ananassa duch.)

被引：12

作者：

Wu, JL

Khan, AA

Shih, CYT

Shih, DS ^{[1
]}

机构：

[1] Louisiana State Univ, Dept Biol Sci, Div Biochem & Mol Biol, Baton Rouge, LA 70803 USA

[2] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA

[3] So Univ, Hlth Res Ctr, RCMI Program, Dept Biol, Baton Rouge, LA 70813 USA

来源：

DNA SEQUENCE | 2001年 / 12卷 / 5-6期

关键词：

strawberry; osmotin-like protein gene; pathogenesis-related proteins; PR-5; proteins; genomic clone;

D O I：

10.3109/10425170109084473

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Osmotin and osmotin-like proteins (OLPs) are pathogenesis-related (PR) proteins, whose synthesis is normally stimulated upon infection of plants by pathogens. A strawberry genomic clone containing an osmotin-like protein (OLP) gene was isolated and sequenced. This clone contains an open reading frame of 681 nucleotides without any intron. The predicted amino acid sequence of the protein shares high degrees of homology with a number of other OLPs and related proteins, of which several are known to have antifungal activities. Southern hybridization analysis of strawberry genomic DNA suggested that the OLP is coded by a multi-gene family. Results from reverse transcriptase-polymerase chain reaction indicated that this OLP gene is expressed in uninfected strawberry plants.

引用

页码：447 / 453

页数：7

共 20 条

[1] Antifungal activity of tobacco osmotin has specificity and involves plasma membrane permeabilization [J].