The carbohydrates of the isoforms of three avian riboflavin-binding proteins

被引:16
作者
Amoresano, A
Brancaccio, A
Andolfo, A
Perduca, M
Monaco, HL
Marino, G
机构
[1] Univ Pavia, Dept Genet, I-27100 Pavia, Italy
[2] Int Mass Spectrometry Facilities Ctr, Naples, Italy
[3] Univ Naples Federico II, Dept Organ & Biol Chem, Naples, Italy
[4] CEINGE, Adv Biotechnol, Naples, Italy
[5] Univ Verona, Fac Sci, Biocrystallog Lab, I-37100 Verona, Italy
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 263卷 / 03期
关键词
riboflavin-binding protein; phosphoglycoprotein; oligosaccharide; structural characterization; MS;
D O I
10.1046/j.1432-1327.1999.00570.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The carbohydrate chains of nine isoforms of chicken egg-white riboflavin-binding protein (RfBP) and six isoforms each of quail egg-white and yolk RfBP have been structurally characterized. The two N-glycosylation sites, Asn36 and Asn147, of the most abundant isoform of each of the three proteins were analyzed in further detail leading to the identification of different glycosylation patterns. In both chicken and quail egg-white RfBP the carbohydrates attached to position 36 had a lower degree of branching and, in the case of the quail protein, this site was only partially glycosylated. A very heterogeneous mixture of complex structures was characteristic of the other glycosylation site. Analysis of the two Sites in quail yolk RfBP confirmed this result which agrees with what has been established for hen yolk RfBP. The presence in the three proteins of a highly heterogeneous mixture of differently branched glycans suggests that the differences in isoelectric points, which is a peculiarity of the different isoforms, are probably indeed due to differences in carbohydrate structure.
引用
收藏
页码:849 / 858
页数:10
相关论文
共 25 条
[1]  
BECVAR J, 1982, J BIOL CHEM, V257, P5607
[2]   Evaluation of quail egg white riboflavin binding protein as a chiral selector in high-performance liquid chromatography and capillary electrophoresis [J].
De Lorenzi, E ;
Massolini, G ;
Lloyd, DK ;
Monaco, HL ;
Galbusera, C ;
Caccialanza, G .
JOURNAL OF CHROMATOGRAPHY A, 1997, 790 (1-2) :47-64
[3]   PREPARATION AND DESORPTION MASS-SPECTROMETRY OF PERMETHYL AND PERACETYL DERIVATIVES OF OLIGOSACCHARIDES [J].
DELL, A .
METHODS IN ENZYMOLOGY, 1990, 193 :647-660
[4]   AFFINITY CHROMATOGRAPHIC PURIFICATION AND COMPARISON OF RIBOFLAVIN-BINDING PROTEINS FROM LAYING HEN LIVER AND BLOOD AND FROM EGG-YOLK [J].
FROEHLICH, JA ;
MERRILL, AH ;
CLAGETT, CO ;
MCCORMICK, DB .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1980, 66 (03) :397-401
[5]   CHARACTERIZATION OF HEN EGG-WHITE RIBOFLAVIN AND YOLK-RIBOFLAVIN BINDING-PROTEINS AND AMINO-ACID-SEQUENCE OF EGG-WHITE RIBOFLAVIN BINDING-PROTEIN [J].
HAMAZUME, Y ;
MEGA, T ;
IKENAKA, T .
JOURNAL OF BIOCHEMISTRY, 1984, 95 (06) :1633-1644
[6]   Identification of oligosaccharides by matrix-assisted laser desorption ionization and electrospray MS [J].
Harvey, DJ ;
Naven, TJP ;
Kuster, B .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1996, 24 (03) :905-912
[7]  
LUMNEY J, 1976, P NATL ACAD SCI USA, V75, P341
[8]  
MANDELES S, 1962, J BIOL CHEM, V237, P3196
[9]   DEVELOPMENT OF A FLAVOPROTEIN COLUMN FOR CHIRAL SEPARATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
MANO, N ;
ODA, Y ;
ASAKAWA, N ;
YOSHIDA, Y ;
SATO, T ;
MIWA, T .
JOURNAL OF CHROMATOGRAPHY, 1992, 623 (02) :221-228
[10]   EGG-YOLK RIBOFLAVIN BINDING-PROTEIN AS A NEW CHIRAL STATIONARY-PHASE IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
MASSOLINI, G ;
DELORENZI, E ;
PONCI, MC ;
GANDINI, C ;
CACCIALANZA, G ;
MONACO, HL .
JOURNAL OF CHROMATOGRAPHY A, 1995, 704 (01) :55-65