A fluorescent indicator to visualize activities of the androgen receptor ligands in single living cells

被引：27

作者：

Awais, M

Sato, M

Lee, XF

Umezawa, Y ^{[1
]}

机构：

[1] Univ Tokyo, Sch Sci, Dept Chem, Bunkyo Ku, Tokyo 1130033, Japan

[2] Japan Sci & Technol Agcy, Bunkyo Ku, Tokyo 1130033, Japan

[3] Japan Sci & Technol Agcy, Kawaguchi, Saitama, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 17期

关键词：

agonists; antagonists; fluorescent probes; FRET (fluorescence resonant energy transfer); receptors;

D O I：

10.1002/anie.200503185

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Seeing the light: An androgen promotes interactions between the androgen-receptor ligand-binding domain (AR LBD) and a coactivator protein. This results in an increase in the fluorescence resonant energy transfer (FRET) from cyan fluorescent protein (CFP) to yellow fluorescent protein (YFP). The indicator distinguishes ligands of different potencies for the AR. (Figure Presented) © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：2707 / 2712

页数：6

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