A fluorescent indicator to visualize activities of the androgen receptor ligands in single living cells

被引：27

作者：

Awais, M

Sato, M

Lee, XF

Umezawa, Y ^{[1
]}

机构：

[1] Univ Tokyo, Sch Sci, Dept Chem, Bunkyo Ku, Tokyo 1130033, Japan

[2] Japan Sci & Technol Agcy, Bunkyo Ku, Tokyo 1130033, Japan

[3] Japan Sci & Technol Agcy, Kawaguchi, Saitama, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 17期

关键词：

agonists; antagonists; fluorescent probes; FRET (fluorescence resonant energy transfer); receptors;

D O I：

10.1002/anie.200503185

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Seeing the light: An androgen promotes interactions between the androgen-receptor ligand-binding domain (AR LBD) and a coactivator protein. This results in an increase in the fluorescence resonant energy transfer (FRET) from cyan fluorescent protein (CFP) to yellow fluorescent protein (YFP). The indicator distinguishes ligands of different potencies for the AR. (Figure Presented) © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：2707 / 2712

页数：6

相关论文

共 31 条

[11] Evaluation of chemicals with endocrine modulating activity in a yeast-based steroid hormone receptor gene transcription assay [J].

Gaido, KW ;

Leonard, LS ;

Lovell, S ;

Gould, JC ;

Babai, D ;

Portier, CJ ;

McDonnell, DP .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1997, 143 (01) :205-212

[12] Tip60 is a co-activator specific for class I nuclear hormone receptors [J].

Gaughan, L ;

Brady, ME ;

Cook, S ;

Neal, DE ;

Robson, CN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (50) :46841-46848

[13] Molecular action of androgens [J].

Gobinet, J ;

Poujol, N ;

Sultan, C .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2002, 198 (1-2) :15-24

[14] FXXLF and WXXLF sequences mediate the NH2-terminal interaction with the ligand binding domain of the androgen receptor [J].

He, B ;

Kemppainen, JA ;

Wilson, EM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (30) :22986-22994

[15]

HERRY DM, 1997, NATURE, V387, P733

[16] Nonsteroidal antiandrogens: a therapeutic option for patients with advanced prostate cancer who wish to retain sexual interest and function [J].

Iversen, P ;

Melezinek, I ;

Schmidt, A .

BJU INTERNATIONAL, 2001, 87 (01) :47-56

[17] Antiandrogenic effects of bisphenol A and nonylphenol on the function of androgen receptor [J].

Lee, HJ ;

Chattopadhyay, S ;

Gong, EY ;

Ahn, RS ;

Lee, K .

TOXICOLOGICAL SCIENCES, 2003, 75 (01) :40-46

[18]

Ma H, 1999, MOL CELL BIOL, V19, P6164

[19] Structural evidence for ligand specificity in the binding domain of the human androgen receptor -: Implications for pathogenic gene mutations [J].

Matias, PM ;

Donner, P ;

Coelho, R ;

Thomaz, M ;

Peixoto, C ;

Macedo, S ;

Otto, N ;

Joschko, S ;

Scholz, P ;

Wegg, A ;

Bäsler, S ;

Schäfer, M ;

Egner, U ;

Carrondo, MA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (34) :26164-26171

[20] Tamoxifen resistance by a conformational arrest of the estrogen receptor α after PKA activation in breast cancer [J].

Michalides, R ;

Griekspoor, A ;

Balkenende, A ;

Verwoerd, D ;

Janssen, L ;

Jalink, K ;

Floore, A ;

Velds, A ;

van 't Veer, L ;

Neefjes, J .

CANCER CELL, 2004, 5 (06) :597-605

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