Reassortant analysis of guinea pig virulence of Pichinde virus variants

被引：28

作者：

Zhang, LH

Marriott, KA

Harnish, DG

Aronson, JF

机构：

[1] Univ Texas, Med Branch, Dept Pathol, WHO Collaborating Ctr Trop Dis, Galveston, TX 77555 USA

[2] McMaster Univ, Dept Pathol, Mol Virol & Immunol Programme, Hamilton, ON L8N 3Z5, Canada

来源：

VIROLOGY | 2001年 / 290卷 / 01期

关键词：

D O I：

10.1006/viro.2001.1127

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The new world arenavirus Pichinde (PIC) is the basis of an accepted small animal model for human Lassa fever. PIC (Munchique strain) variant P2 is attenuated in guinea pigs, whereas variant P18 is extremely virulent. Previous sequence analysis of the S segments of these two viruses indicated a small number of possible virulence markers in the glycoprotein precursor (GPC) and nucleoprotein (NP) genes. In order to determine the role of these S segment genes in guinea pig virulence in this system, we have generated reassortant viruses. When tested in outbred guinea pigs, the reassortant containing the S segment from the virulent parent P18 (S18L2) caused significantly higher morbidity than the reciprocal reassortant. This increased morbidity was associated with higher viral titers in serum and spleen. However, the S18L2 reassortant was not as fully virulent in this system as the P18 parent, indicating a role for L segment genes in virulence. (C) 2001 Academic Press.

引用

页码：30 / 38

页数：9

共 29 条

[1] MOLECULAR-BASIS OF ORGAN-SPECIFIC SELECTION OF VIRAL VARIANTS DURING CHRONIC INFECTION [J].