The first complete genome sequences of clinical isolates of human coronavirus 229E

被引：32

作者：

Farsani, Seyed Mohammad Jazaeri ^{[1
,2
]}

Dijkman, Ronald ^{[1
]}

Jebbink, Maarten F. ^{[1
]}

Goossens, Herman ^{[3
]}

Ieven, Margareta ^{[3
]}

Deijs, Martin ^{[1
]}

Molenkamp, Richard ^{[4
]}

van der Hoek, Lia ^{[1
]}

机构：

[1] Univ Amsterdam, Acad Med Ctr, Dept Med Microbiol, Lab Expt Virol,Ctr Infect & Immun Amsterdam CINIM, NL-1105 AZ Amsterdam, Netherlands

[2] Univ Tehran Med Sci, Tehran, Iran

[3] Univ Antwerp, Univ Antwerp Hosp, Vaccine & Infect Dis Inst, Dept Med Microbiol, B-2020 Antwerp, Belgium

[4] Univ Amsterdam, Acad Med Ctr, Dept Med Microbiol, Lab Clin Virol,Ctr Infect & Immun Amsterdam CINIM, NL-1105 AZ Amsterdam, Netherlands

来源：

VIRUS GENES | 2012年 / 45卷 / 03期

关键词：

Human coronavirus 229E; Respiratory tract infection; Complete genome sequence; Spike gene; ORF4; gene; Nucleocapsid gene; 3' UNTRANSLATED REGION; RESPIRATORY-TRACT; RNA; VIRUS; SPIKE; REPLICATION; HCOV-229E; PROTEINS; ENCODES; FUSION;

D O I：

10.1007/s11262-012-0807-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Human coronavirus 229E has been identified in the mid-1960s, yet still only one full-genome sequence is available. This full-length sequence has been determined from the cDNA-clone Inf-1 that is based on the lab-adapted strain VR-740. Lab-adaptation might have resulted in genomic changes, due to insufficient pressure to maintain gene integrity of non-essential genes. We present here the first full-length genome sequence of two clinical isolates. Each encoded gene was compared to Inf-1. In general, little sequence changes were noted, most could be attributed to genetic drift, since the clinical isolates originate from 2009 to 2010 and VR740 from 1962. Hot spots of substitutions were situated in the S1 region of the Spike, the nucleocapsid gene, and the non-structural protein 3 gene, whereas several deletions were detected in the 3'UTR. Most notable was the difference in genome organization: instead of an ORF4A and ORF4B, an intact ORF4 was present in clinical isolates.

引用

页码：433 / 439

页数：7

共 24 条

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