Beyond silencing - engineering applications of RNA interference and antisense technology for altering cellular phenotype

被引：38

作者：

Hebert, Colin G. ^{[1
,2
,4
]}

Valdes, James J. ^{[2
]}

Bentley, William E. ^{[1
,3
,4
]}

机构：

[1] Univ Maryland, Inst Biotechnol, Ctr Biosyst Res, College Pk, MD 20742 USA

[2] USA, Edgewood Chem Biol Ctr, AMSRD ECB AP B Michel E3330, Aberdeen Proving Ground, MD 21010 USA

[3] Univ Maryland, Dept Chem & Biomol Engn, College Pk, MD 20742 USA

[4] Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2008年 / 19卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.copbio.2008.08.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Since its discovery 10 years ago, RNA interference (RNAi) has evolved from a research tool into a powerful method for altering the phenotype of cells and whole organisms. Its near universal applicability coupled with its pinpoint accuracy for suppressing target proteins has altered the landscape of many fields. While there is considerable intellectual investment in therapeutics, its potential extends far beyond. In this review, we explore some of these emerging applications - metabolic engineering for enhancing recombinant protein production in both insect and mammalian cell systems, antisense technologies in bacteria as next generation antibiotics, and RNAi in plant biotechnology for improving productivity and nutritional value.

引用

页码：500 / 505

页数：6

共 59 条

[11] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[12] Initial identification of low temperature and culture stage induction of miRNA expression in suspension CHO-K1 cells [J].

Gammell, Patrick ;

Barron, Niall ;

Kumar, Niraj ;

Clynes, Martin .

JOURNAL OF BIOTECHNOLOGY, 2007, 130 (03) :213-218

[13]

Geller BL, 2005, CURR OPIN MOL THER, V7, P109

[14] Antisense antibacterials [J].

Good, L .

EXPERT OPINION ON THERAPEUTIC PATENTS, 2002, 12 (08) :1173-1179

[15] ARTIFICIAL REGULATION OF GENE-EXPRESSION IN ESCHERICHIA-COLI BY RNASE-P [J].

GUERRIERTAKADA, C ;

LI, Y ;

ALTMAN, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) :11115-11119

[16] Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells [J].

Hacker, DL ;

Bertschinger, M ;

Baldi, L ;

Wurm, FM .

GENE, 2004, 341 :227-234

[17] Hairpin extensions enhance the efficacy of mycolyl transferase-specific antisense oligonucleotides targeting Mycobacterium tuberculosis [J].

Harth, Gunter ;

Zamecnik, Paul C. ;

Tabatadze, David ;

Pierson, Katherine ;

Horwitz, Marcus A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (17) :7199-7204

[18] High-throughput vectors for efficient gene silencing in plants [J].

Helliwell, CA ;

Wesley, SV ;

Wielopolska, AJ ;

Waterhouse, PM .

FUNCTIONAL PLANT BIOLOGY, 2002, 29 (10) :1217-1225

[19] High-lysine corn generated by endosperm-specific suppression of lysine catabolism using RNAi [J].

Hournard, Nancy M. ;

Mainville, Jonnelle L. ;

Bonin, Christopher P. ;

Huang, Shihshieh ;

Luethy, Michael H. ;

Malvar, Thomas M. .

PLANT BIOTECHNOLOGY JOURNAL, 2007, 5 (05) :605-614

[20]

IMAINISHIYA H, 2007, BMC BIOTECHNOL, P7

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