Potassium release, a useful tool for studying antimicrobial peptides

被引：74

作者：

Orlov, DS ^{[1
]}

Nguyen, T ^{[1
]}

Lehrer, RI ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Hlth Sci Ctr, Dept Med, Los Angeles, CA 90095 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2002年 / 49卷 / 03期

关键词：

electrode; bacteria; potassium;

D O I：

10.1016/S0167-7012(01)00383-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We used an ion-selective electrode to measure potassium release from bacteria treated with antimicrobial peptides. This broadly applicable and simple technique for assessing membrane integrity deserves greater use. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：325 / 328

页数：4

共 10 条

[1] Potassium ion efflux induced by cationic compounds in yeast [J].

Enríquez-Freire, E ;

López, R ;

Peña, A .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1999, 1418 (01) :147-157

[2] MODE OF ACTION OF GRAMICIDIN-S ON ESCHERICHIA-COLI MEMBRANE [J].

KATSU, T ;

KOBAYASHI, H ;

FUJITA, Y .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 860 (03) :608-619

[3] PROTEGRINS - LEUKOCYTE ANTIMICROBIAL PEPTIDES THAT COMBINE FEATURES OF CORTICOSTATIC DEFENSINS AND TACHYPLESINS [J].

KOKRYAKOV, VN ;

HARWIG, SSL ;

PANYUTICH, EA ;

SHEVCHENKO, AA ;

ALESHINA, GM ;

SHAMOVA, OV ;

KORNEVA, HA ;

LEHRER, RI .

FEBS LETTERS, 1993, 327 (02) :231-236

[4] CONCURRENT ASSESSMENT OF INNER AND OUTER-MEMBRANE PERMEABILIZATION AND BACTERIOLYSIS IN ESCHERICHIA-COLI BY MULTIPLE-WAVELENGTH SPECTROPHOTOMETRY [J].

LEHRER, RI ;

BARTON, A ;

GANZ, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 108 (1-2) :153-158

[5] Interactions of an antimicrobial peptide, magainin 2, with outer and inner membranes of Gram-negative bacteria [J].

Matsuzaki, K ;

Sugishita, K ;

Harada, M ;

Fujii, N ;

Miyajima, K .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1327 (01) :119-130

[6] Determination of potassium flux activity of viable human erythrocytes by measuring the release-influx ratio [J].

Ozawa, Y ;

Sato, S ;

Matsumoto, K ;

Nishi, S ;

Shishido, S ;

Imafuku, Y ;

Miura, Y ;

Yoshida, H .

CLINICA CHIMICA ACTA, 1999, 279 (1-2) :125-132

[7] Measurements of net fluxes and extracellular changes of H+, Ca2+, K+, and NH4+ in Escherichia coli using ion-selective microelectrodes [J].

Shabala, L ;

Ross, T ;

Newman, I ;

McMeekin, T ;

Shabala, S .

JOURNAL OF MICROBIOLOGICAL METHODS, 2001, 46 (02) :119-129

[8] Membrane destabilization assay based on potassium release from liposomes [J].

Silberstein, A ;

Mirzabekov, T ;

Anderson, WF ;

Rozenberg, Y .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1999, 1461 (01) :103-112

[9] SMAP-29: a potent antibacterial and antifungal peptide from sheep leukocytes [J].

Skerlavaj, B ;

Benincasa, M ;

Risso, A ;

Zanetti, M ;

Gennaro, R .

FEBS LETTERS, 1999, 463 (1-2) :58-62

[10] Membrane channel formation by antimicrobial protegrins [J].

Sokolov, Y ;

Mirzabekov, T ;

Martin, DW ;

Lehrer, RI ;

Kagan, BL .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1999, 1420 (1-2) :23-29

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