Preparation and crystallization of selenomethionyl dextranase from Penicillium minioluteum expressed in Pichia pastoris

被引：24

作者：

Larsson, AM

Ståhlberg, J

Jones, TA

机构：

[1] Uppsala Univ, Ctr Biomed, Dept Cell & Mol Biol, SE-75124 Uppsala, Sweden

[2] Swedish Univ Agr Sci, Ctr Biomed, Dept Mol Biol, SE-75124 Uppsala, Sweden

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷

关键词：

D O I：

10.1107/S0907444901020406

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Dextranase from the fungus Penicillium minioluteum hydrolyses alpha-1,6-glycosidic bonds in dextran polymers. The enzyme has been expressed in Pichia pastoris in the presence of selenomethionine (SeMet). The level of SeMet incorporation was estimated by amino-acid analysis to be 50%. The protein has been crystallized in space group P2(1)2(1)2, with unit-cell parameters a = 103.6, b = 115.3, c = 49.8 Angstrom and one molecule per asymmetric unit. The crystals diffract to 2.0 Angstrom and the presence of SeMet in the crystals has been confirmed by an X-ray absorption spectrum.

引用

页码：346 / 348

页数：3

共 15 条

[1] Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II [J].