Halogenation of unactivated carbon centers in natural product biosynthesis: Trichlorination of leucine during barbamide biosynthesis

被引：143

作者：

Galonic, DP ^{[1
]}

Vaillancourt, FH ^{[1
]}

Walsh, CT ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 12期

关键词：

D O I：

10.1021/ja060151n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The in vitro reconstitution of leucine halogenation during barbamide biosynthesis has been accomplished. It has been demonstrated that the triple chlorination of the unactivated pro-R methyl group of the peptidyl carrier protein-tethered L-Leu substrate is carried out by the tandem action of two nonheme iron(II)-dependent halogenases, BarB1 and BarB2. Investigation of the substrate specificities of each of the halogenating enzymes revealed their complementary roles in the generation of trichloroleucine. Copyright © 2006 American Chemical Society.

引用

页码：3900 / 3901

页数：2

共 23 条

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