Sulfated galactofucan from Lobophora variegata:: Anticoagulant and anti-inflammatory properties

被引：63

作者：

Medeiros, V. P. ^{[1
]}

Queiroz, K. C. S. ^{[2
]}

Cardoso, M. L. ^{[1
]}

Monteiro, G. R. G. ^{[1
]}

Oliveira, F. W. ^{[1
]}

Chavante, S. F. ^{[1
]}

Guimaraes, L. A. ^{[1
]}

Rocha, H. A. O. ^{[1
]}

Leite, E. L. ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Norte, Ctr Biociencias, Dept Bioquim, BR-59072970 Natal, RN, Brazil

[2] Univ Fed Rio Grande do Norte, Dept Biofis & Farmacol, BR-59072970 Natal, RN, Brazil

来源：

BIOCHEMISTRY-MOSCOW | 2008年 / 73卷 / 09期

关键词：

brown algae; Lobophora variegata; fucan; fucoidan; anti-clotting activity; anti-inflammatory activity;

D O I：

10.1134/S0006297908090095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sulfated polysaccharides (fucans and fucoidans) from brown algae show several biological activities, including anticoagulant and anti-inflammatory activities. We have extracted a sulfated heterofucan from the brown seaweed Lobophora variegata by proteolytic digestion, followed by acetone fractionation, molecular sieving, and ion-exchange chromatography. Chemical analyses and C-13-NMR and IR spectroscopy showed that this fucoidan is composed of fucose, galactose, and sulfate at molar ratios of 1:3:2. We compared the anticoagulant activity of L. variegata fucoidan with those of a commercial sulfated polysaccharide (also named fucoidan) from Fucus vesiculosus and heparin. The experimental inflammation models utilized in this work revealed that fucoidan from L. variegata inhibits leukocyte migration to the inflammation site. Ear swelling caused by croton oil was also inhibited when sulfated polysaccharides from F. vesiculosus and L. variegata were used. The precise mechanism of different action between homo-and heterofucans is not clear; nevertheless, the polysaccharides studied here may have therapeutic potential in inflammatory disorders.

引用

页码：1018 / 1024

页数：7

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