Determination of catecholamines in human plasma by high-performance liquid chromatography with electrochemical detection

被引：100

作者：

Raggi, MA

Sabbioni, C

Casamenti, G

Gerra, G

Calonghi, N

Masotti, L

机构：

[1] Univ Bologna, Dept Pharmaceut Sci, I-40126 Bologna, Italy

[2] AUSL Parma, Ser T, I-43100 Santo Domingo, Italy

[3] Univ Bologna, Dept Biochem G Moruzzi, I-40126 Bologna, Italy

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 730卷 / 02期

关键词：

catecholamines;

D O I：

10.1016/S0378-4347(99)00213-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, assays were improved for the determination of catecholamines in human plasma. High-performance liquid chromatography with electrochemical detection was employed for quantitative analysis. The influence of various parameters on chromatographic performance, such as the composition and the pH of the mobile phase, and the detection potential, was investigated. An accurate solid-phase extraction procedure, after catecholamine complexation with diphenylborate, was developed. The efficiency yield for all catecholamines was in the range 92-98%. Relative standard deviation values for repeatability and for intermediate precision were less than 2% and 3%, respectively, for all three analytes. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：201 / 211

页数：11

共 31 条

[1] EFFECTS OF MOBILE PHASE-COMPOSITION ON THE CHROMATOGRAPHIC AND ELECTROCHEMICAL-BEHAVIOR OF CATECHOLAMINES AND SELECTED METABOLITES - REVERSED-PHASE ION-PAIRED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING MULTIPLE-ELECTRODE DETECTION [J].