Boosting with an adenovirus-based vaccine improves protective efficacy against Venezuelan equine encephalitis virus following DNA vaccination

被引：21

作者：

Perkins, SD ^{[1
]}

O'Brien, LM ^{[1
]}

Phillpotts, RJ ^{[1
]}

机构：

[1] Def Sci & Technol Lab, Dept Biomed Sci, Salisbury SP4 0JQ, Wilts, England

来源：

VACCINE | 2006年 / 24卷 / 17期

关键词：

DNA vaccine; prime-boost; VEEV; Venezuelan equine encephalitis virus; adenovirus;

D O I：

10.1016/j.vaccine.2006.02.020

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

There is a requirement for a vaccine that protects against the alphavirus, Venezuelan equine encephalitis virus (VEEV). Previous work has shown that DNA vaccines encoding structural proteins of VEEV can elicit immune responses and protection against VEEV though this protection is incomplete against airborne VEEV. In this study, we demonstrate that particle-mediated epidermal delivery of a DNA vaccine encoding the E2 glycoprotein of VEEV can be boosted with a mucosally-delivered Ad-based vaccine encoding the same E2 glycoprotein. This results in an improved Th2-type IgG response, an increase in neutralising antibody and a significant increase in protection against airborne VEEV. This indicates that prime-boost may be a suitable immunisation regimen for providing protection against airborne VEEV. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：3440 / 3445

页数：6

共 39 条

[1]

ALIBEK K, 1999, EMERGING BW THREAT L

[2] Control of a mucosal challenge and prevention of AIDS by a multiprotein DNA/MVA vaccine [J].

Amara, RR ;

Villinger, F ;

Altman, JD ;

Lydy, SL ;

O'Neil, SP ;

Staprans, SI ;

Montefiori, DC ;

Xu, Y ;

Herndon, JG ;

Wyatt, LS ;

Candido, MA ;

Kozyr, NL ;

Earl, PL ;

Smith, JM ;

Ma, HL ;

Grimm, BD ;

Hulsey, ML ;

Miller, J ;

McClure, HM ;

McNicholl, JM ;

Moss, B ;

Robinson, HL .

SCIENCE, 2001, 292 (5514) :69-74

[3] Gene gun mediated vaccination is superior to manual delivery for immunisation with DNA vaccines expressing protective antigens from Yersinia pestis or Venezuelan Equine Encephalitis virus [J].

Bennett, AM ;

Phillpotts, RJ ;

Perkins, SD ;

Jacobs, SC ;

Williamson, ED .

VACCINE, 1999, 18 (7-8) :588-596

[4]

Bennett GJ, 2000, J PAIN SYMPTOM MANAG, V19, pS2

[5] Induction of Plasmodium falciparum transmission-blocking antibodies in nonhuman primates by a combination of DNA and protein immunizations [J].

Coban, C ;

Philipp, MT ;

Purcell, JE ;

Keister, DB ;

Okulate, M ;

Martin, DS ;

Kumar, N .

INFECTION AND IMMUNITY, 2004, 72 (01) :253-259

[6] The role of particle-mediated DNA vaccines in biodefense preparedness [J].

Dean, HJ ;

Haynes, J ;

Schmaljohn, C .

ADVANCED DRUG DELIVERY REVIEWS, 2005, 57 (09) :1315-1342

[7] 2 HUMAN CASES OF LABORATORY INFECTION WITH MUCAMBO VIRUS [J].

DEMUCHAMACIAS, J ;

SANCHEZS.I .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1965, 14 (03) :475-+

[8] 10 CLINICAL CASES OF HUMAN INFECTION WITH VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS, SUBTYPE-I-D [J].

DIETZ, WH ;

PERALTA, PH ;

JOHNSON, KM .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1979, 28 (02) :329-334

[9] Role for mucosal immune responses and cell-mediated immune functions in protection from airborne challenge with Venezuelan equine encephalitis virus [J].

Elvin, SJ ;

Bennett, AM ;

Phillpotts, RJ .

JOURNAL OF MEDICAL VIROLOGY, 2002, 67 (03) :384-393

[10] MEASUREMENT OF THE RESPIRATORY VOLUMES OF LABORATORY ANIMALS [J].

GUYTON, AC .

AMERICAN JOURNAL OF PHYSIOLOGY, 1947, 150 (01) :70-77

← 1 2 3 4 →