Immature and Mature Dengue Serotype 1 Virus Structures Provide Insight into the Maturation Process

被引：114

作者：

Kostyuchenko, Victor A. ^{[1
,2
]}

Zhang, Qian ^{[1
,2
]}

Tan, Joanne L. ^{[1
,2
]}

Ng, Thiam-Seng ^{[1
,2
]}

Lok, Shee-Mei ^{[1
,2
]}

机构：

[1] Duke NUS Grad Med Sch, Program Emerging Infect Dis, Singapore, Singapore

[2] Natl Univ Singapore, Ctr Bioimaging Sci, Singapore 117548, Singapore

来源：

JOURNAL OF VIROLOGY | 2013年 / 87卷 / 13期

关键词：

ENVELOPE PROTEIN; ELECTRON-MICROSCOPY; MOLECULAR-DYNAMICS; CORE PROTEIN; VISUALIZATION; GLYCOPROTEIN; RESOLUTION; INFECTION; ANTIBODY; BINDING;

D O I：

10.1128/JVI.00197-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Dengue virus is a major human pathogen that has four serotypes (DENV1 to -4). Here we report the cryoelectron microscopy (cryo-EM) structures of immature and mature DENV1 at 6- and 4.5-angstrom resolution, respectively. The subnanometer-resolution maps allow accurate placement of all of the surface proteins. Although the immature and mature viruses showed vastly different surface protein organizations, the envelope protein transmembrane (E-TM) regions remain in similar positions. The pivotal role of the E-TM regions leads to the identification of the start and end positions of all surface proteins during maturation.

引用

页码：7700 / 7707

页数：8

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