Real-Time Dynamics of RNA Polymerase II Clustering in Live Human Cells

被引：373

作者：

Cisse, Ibrahim I. ^{[1
,2
,3
]}

Izeddin, Ignacio ^{[1
,2
]}

Causse, Sebastien Z. ^{[1
]}

Boudarene, Lydia ^{[1
,2
]}

Senecal, Adrien ^{[1
,6
]}

Muresan, Leila ^{[4
]}

Dugast-Darzacq, Claire ^{[1
,5
]}

Hajj, Bassam ^{[3
]}

Dahan, Maxime ^{[2
,3
,6
]}

Darzacq, Xavier ^{[1
,3
]}

机构：

[1] Ecole Normale Super, Inst Biol, IBENS, CNRS,UMR8197, F-75005 Paris, France

[2] Ecole Normale Super, Inst Biol, Dept Phys, Lab Kastler Brossel,CNRS,UMR8552, F-75230 Paris 05, France

[3] Transcript Imaging Consortium, Ashburn, VA 20147 USA

[4] Ecole Normale Super, Inst Biol, IBENS, DYOGEN,CNRS,UMR8197, F-75005 Paris, France

[5] Univ Paris Diderot, Paris Cite Sorbonne, F-75013 Paris, France

[6] Univ Paris 06, F-75005 Paris, France

来源：

SCIENCE | 2013年 / 341卷 / 6146期

关键词：

IN-VIVO; LIVING CELLS; GENE-EXPRESSION; P-TEFB; TRANSCRIPTION; ORGANIZATION; PROTEIN; REPLICATION; ELONGATION; MECHANISMS;

D O I：

10.1126/science.1239053

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Transcription is reported to be spatially compartmentalized in nuclear transcription factories with clusters of RNA polymerase II (Pol II). However, little is known about when these foci assemble or their relative stability. We developed a quantitative single-cell approach to characterize protein spatiotemporal organization, with single-molecule sensitivity in live eukaryotic cells. We observed that Pol II clusters form transiently, with an average lifetime of 5.1 (+/- 0.4) seconds, which refutes the notion that they are statically assembled substructures. Stimuli affecting transcription yielded orders-of-magnitude changes in the dynamics of Pol II clusters, which implies that clustering is regulated and plays a role in the cell's ability to effect rapid response to external signals. Our results suggest that transient crowding of enzymes may aid in rate-limiting steps of gene regulation.

引用

页码：664 / 667

页数：4

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