Cloning and expression of the human N-methyl-D-aspartate receptor subunit NR3A

被引：48

作者：

Eriksson, M ^{[1
]}

Nilsson, A ^{[1
]}

Froelich-Fabre, S ^{[1
]}

Åkesson, E ^{[1
]}

Dunker, J ^{[1
]}

Seiger, Å ^{[1
]}

Folkesson, R ^{[1
]}

Benedikz, E ^{[1
]}

Sundström, E ^{[1
]}

机构：

[1] Karolinska Inst, NEUROTEC, KFC Novum, Sect Expt Geriat, S-14186 Huddinge, Sweden

来源：

NEUROSCIENCE LETTERS | 2002年 / 321卷 / 03期

关键词：

glutamate receptor; human; cloning; messenger RNA;

D O I：

10.1016/S0304-3940(01)02524-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Native N-methyl-D-aspartate (NMDA) receptors are heteromeric assemblies of four or five subunits. The NMDA receptor subunits, NR1, NR2A, NR2B, NR2C, and NR2D have been cloned in several species, including man. The NR3A subunit, which in rodents is predominantly expressed during early development, seems to function by reducing the NMDA receptor response. The human homologue to the rat NR3A, however, had not been cloned. In order to study the functions of the human NR3A (hNR3A), we have cloned and sequenced the hNR3A. It was found to share 88% of the DNA sequence with the rat gene, corresponding to a 93% homology at the amino acid level. Based on available data from human genome databases, we localized the gene to chromosome 9. The transcript could be detected by in situ hybridization in human fetal spinal cord and forebrain. Two splice variants of NR3A have been reported in rat brain, the longer of the two containing a 60 bp insert in the intracellular domain. We were unable to detect this 60 bp insert in fetal or adult human brain, suggesting that only the short variant is expressed in humans. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：177 / 181

页数：5

共 9 条

[1] STABLE TRANSFECTION OF THE NR1 SUBUNIT IN CHINESE-HAMSTER OVARY CELLS FAILS TO PRODUCE A FUNCTIONAL N-METHYL-D-ASPARTATE RECEPTOR [J].