Mammalian 5′-Capped MicroRNA Precursors that Generate a SingleMicroRNA

被引：190

作者：

Xie, Mingyi ^{[1
]}

Li, Mingfeng ^{[2
]}

Vilborg, Anna ^{[1
]}

Lee, Nara ^{[1
]}

Shu, Mei-Di ^{[1
]}

Yartseva, Valeria ^{[3
]}

Sestan, Nenad ^{[2
]}

Steitz, Joan A. ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Boyer Ctr Mol Med, Dept Mol Biophys & Biochem,Howard Hughes Med Inst, New Haven, CT 06536 USA

[2] Yale Univ, Sch Med, Dept Neurobiol, Kavli Inst Neurosci, New Haven, CT 06510 USA

[3] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06510 USA

来源：

CELL | 2013年 / 155卷 / 07期

基金：

美国国家卫生研究院;

关键词：

RNA-POLYMERASE-II; EMBRYONIC STEM-CELLS; NUCLEAR EXPORT; HERPESVIRUS USES; VIRAL MICRORNAS; MESSENGER-RNAS; IN-VIVO; BIOGENESIS; PATHWAY; EXPRESSION;

D O I：

10.1016/j.cell.2013.11.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) are short RNA gene regulators typically produced from primary transcripts that are cleaved by the nuclear microprocessor complex, with the resulting precursor miRNA hairpins exported by exportin 5 and processed by cytoplasmic Dicer to yield two (5p and 3p) miRNAs. Here, we document microprocessor-independent 7-methylguanosine (m(7)G)-capped pre-miRNAs, whose 5' ends coincide with transcription start sites and 3' ends are most likely generated by transcription termination. By establishing a small RNA Cap-seq method that employs the cap-binding protein eIF4E, we identified a group of murine m(7)G-capped pre-miRNAs genome wide. The m(7)G-capped pre-miRNAs are exported via the PHAX-exportin 1 pathway. After Dicer cleavage, only the 3p-miRNA is efficiently loaded onto Argonaute to form a functional microRNP. This unusual miRNA biogenesis pathway, which differs in pre-miRNA synthesis, nuclear-cytoplasmic transport, and guide strand selection, enables the development of shRNA expression constructs that produce a single 3p-siRNA.

引用

页码：1568 / 1580

页数：13

共 58 条

[1] Mouse ES cells express endogenous shRNAs, siRNAs, and other Microprocessor-independent, Dicer-dependent small RNAs [J].

Babiarz, Joshua E. ;

Ruby, J. Graham ;

Wang, Yangming ;

Bartel, David P. ;

Blelloch, Robert .

GENES & DEVELOPMENT, 2008, 22 (20) :2773-2785

[2] Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II [J].

Baillat, D ;

Hakimi, MA ;

Näär, AM ;

Shilatifard, A ;

Cooch, N ;

Shiekhattar, R .

CELL, 2005, 123 (02) :265-276

[3] A Mammalian Herpesvirus Uses Noncanonical Expression and Processing Mechanisms to Generate Viral MicroRNAs [J].

Bogerd, Hal P. ;

Karnowski, Heather W. ;

Cai, Xuezhong ;

Shin, Jinwook ;

Pohlers, Michael ;

Cullen, Bryan R. .

MOLECULAR CELL, 2010, 37 (01) :135-142

[4] Reprogramming of the tumour microenvironment by stromal PTEN-regulated miR-320 [J].

Bronisz, A. ;

Godlewski, J. ;

Wallace, J. A. ;

Merchant, A. S. ;

Nowicki, M. O. ;

Mathsyaraja, H. ;

Srinivasan, R. ;

Trimboli, A. J. ;

Martin, C. K. ;

Li, F. ;

Yu, L. ;

Fernandez, S. A. ;

Pecot, T. ;

Rosol, T. J. ;

Cory, S. ;

Hallett, M. ;

Park, M. ;

Piper, M. G. ;

Marsh, C. B. ;

Yee, L. D. ;

Jimenez, R. E. ;

Nuovo, G. ;

Lawler, S. E. ;

Chiocca, E. A. ;

Leone, G. ;

Ostrowski, M. C. .

NATURE CELL BIOLOGY, 2012, 14 (02) :159-167

[5] The nuclear export receptor XPO-1 supports primary miRNA processing in C. elegans and Drosophila [J].

Buessing, Ingo ;

Yang, Jr-Shiuan ;

Lai, Eric C. ;

Grosshans, Helge .

EMBO JOURNAL, 2010, 29 (11) :1830-1839

[6] A comprehensive survey of 3′ animal miRNA modification events and a possible role for 3′ adenylation in modulating miRNA targeting effectiveness [J].

Burroughs, A. Maxwell ;

Ando, Yoshinari ;

de Hoon, Michiel J. L. ;

Tomaru, Yasuhiro ;

Nishibu, Takahiro ;

Ukekawa, Ryo ;

Funakoshi, Taku ;

Kurokawa, Tsutomu ;

Suzuki, Harukazu ;

Hayashizaki, Yoshihide ;

Daub, Carsten O. .

GENOME RESEARCH, 2010, 20 (10) :1398-1410

[7] Genome-wide analysis of mammalian promoter architecture and evolution [J].

Carninci, Piero ;

Sandelin, Albin ;

Lenhard, Boris ;

Katayama, Shintaro ;

Shimokawa, Kazuro ;

Ponjavic, Jasmina ;

Semple, Colin A. M. ;

Taylor, Martin S. ;

Engström, Par G. ;

Frith, Martin C. ;

Forrest, Alistair R. R. ;

Alkema, Wynand B. ;

Tan, Sin Lam ;

Plessy, Charles ;

Kodzius, Rimantas ;

Ravasi, Timothy ;

Kasukawa, Takeya ;

Fukuda, Shiro ;

Kanamori-Katayama, Mutsumi ;

Kitazume, Yayoi ;

Kawaji, Hideya ;

Kai, Chikatoshi ;

Nakamura, Mari ;

Konno, Hideaki ;

Nakano, Kenji ;

Mottagui-Tabar, Salim ;

Arner, Peter ;

Chesi, Alessandra ;

Gustincich, Stefano ;

Persichetti, Francesca ;

Suzuki, Harukazu ;

Grimmond, Sean M. ;

Wells, Christine A. ;

Orlando, Valerio ;

Wahlestedt, Claes ;

Liu, Edison T. ;

Harbers, Matthias ;

Kawai, Jun ;

Bajic, Vladimir B. ;

Hume, David A. ;

Hayashizaki, Yoshihide .

NATURE GENETICS, 2006, 38 (06) :626-635

[8] CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs [J].

Castanotto, Daniela ;

Lingeman, Robert ;

Riggs, Arthur D. ;

Rossi, John J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (51) :21655-21659

[9] A Primate Herpesvirus Uses the Integrator Complex to Generate Viral MicroRNAs [J].

Cazalla, Demian ;

Xie, Mingyi ;

Steitz, Joan A. .

MOLECULAR CELL, 2011, 43 (06) :982-992

[10] A Dicer-independent miRNA biogenesis pathway that requires Ago catalysis [J].

Cheloufi, Sihem ;

Dos Santos, Camila O. ;

Chong, Mark M. W. ;

Hannon, Gregory J. .

NATURE, 2010, 465 (7298) :584-U76

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