RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes

被引：78

作者：

Bleeker, Petra M. ^{[1
,2
]}

Spyropoulou, Eleni A. ^{[1
]}

Diergaarde, Paul J. ^{[2
]}

Volpin, Hanne ^{[2
]}

De Both, Michiel T. J. ^{[2
]}

Zerbe, Philipp ^{[3
]}

Bohlmann, Joerg ^{[3
]}

Falara, Vasiliki ^{[4
]}

Matsuba, Yuki ^{[4
]}

Pichersky, Eran ^{[4
]}

Haring, Michel A. ^{[1
]}

Schuurink, Robert C. ^{[1
]}

机构：

[1] Swammerdam Inst Life Sci, Dept Plant Physiol, NL-1098 XH Amsterdam, Netherlands

[2] KeyGene NV, NL-6700 AE Wageningen, Netherlands

[3] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada

[4] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA

来源：

PLANT MOLECULAR BIOLOGY | 2011年 / 77卷 / 4-5期

基金：

美国国家科学基金会;

关键词：

Sesquiterpene synthase; Trichomes; RNA-seq; Wild and cultivated tomato; Jasmonate; MULTIPRODUCT TERPENE SYNTHASE; MEDICAGO-TRUNCATULA; GLANDULAR TRICHOMES; ESCHERICHIA-COLI; GENE FAMILY; TOMATO; BIOSYNTHESIS; WILD; MONOTERPENES; EXPRESSION;

D O I：

10.1007/s11103-011-9813-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Solanum lycopersicum and Solanum habrochaites (f. typicum) accession PI127826 emit a variety of sesquiterpenes. To identify terpene synthases involved in the production of these volatile sesquiterpenes, we used massive parallel pyrosequencing (RNA-seq) to obtain the transcriptome of the stem trichomes from these plants. This approach resulted initially in the discovery of six sesquiterpene synthase cDNAs from S. lycopersicum and five from S. habrochaites. Searches of other databases and the S. lycopersicum genome resulted in the discovery of two additional sesquiterpene synthases expressed in trichomes. The sesquiterpene synthases from S. lycopersicum and S. habrochaites have high levels of protein identity. Several of them appeared to encode for non-functional proteins. Functional recombinant proteins produced germacrenes, beta-caryophyllene/alpha-humulene, viridiflorene and valencene from (E,E)-farnesyl diphosphate. However, the activities of these enzymes do not completely explain the differences in sesquiterpene production between the two tomato plants. RT-qPCR confirmed high levels of expression of most of the S. lycopersicum sesquiterpene synthases in stem trichomes. In addition, one sesquiterpene synthase was induced by jasmonic acid, while another appeared to be slightly repressed by the treatment. Our data provide a foundation to study the evolution of terpene synthases in cultivated and wild tomato.

引用

页码：323 / 336

页数：14

共 58 条

[21] The toxicity of recombinant proteins in Escherichia coli:: A comparison of overexpression in BL21(DE3), C41(DE3), and C43(DE3) [J].

Dumon-Seignovert, L ;

Cariot, G ;

Vuillard, L .

PROTEIN EXPRESSION AND PURIFICATION, 2004, 37 (01) :203-206

[22]

Falara V, PLANT PHYSL IN PRESS

[23] Transcriptome analysis approaches for the isolation of trichome-specific genes from the medicinal plant Cistus creticus subsp creticus [J].

Falara, Vasiliki ;

Fotopoulos, Vasileios ;

Margaritis, Thanasis ;

Anastasaki, Thalia ;

Pateraki, Irene ;

Bosabalidis, Artemios M. ;

Kafetzopoulos, Dimitris ;

Demetzos, Costas ;

Pichersky, Eran ;

Kanellis, Angelos K. .

PLANT MOLECULAR BIOLOGY, 2008, 68 (06) :633-651

[24] Conformational analysis of (+)-germacrene A by variable-temperature NMR and NOE spectroscopy [J].

Faraldos, Juan A. ;

Wu, Shuiqin ;

Chappell, Joe ;

Coates, Robert M. .

TETRAHEDRON, 2007, 63 (32) :7733-7742

[25] Metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species Lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketones [J].

Fridman, E ;

Wang, JH ;

Iijima, Y ;

Froehlich, JE ;

Gang, DR ;

Ohlrogge, J ;

Pichersky, E .

PLANT CELL, 2005, 17 (04) :1252-1267

[26] A Multiproduct Terpene Synthase from Medicago truncatula Generates Cadalane Sesquiterpenes via Two Different Mechanisms [J].

Garms, Stefan ;

Koellner, Tobias G. ;

Boland, Wilhelm .

JOURNAL OF ORGANIC CHEMISTRY, 2010, 75 (16) :5590-5600

[27] Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes [J].

Goepfert, Jens C. ;

MacNevin, Gillian ;

Ro, Dae-Kyun ;

Spring, Otmar .

BMC PLANT BIOLOGY, 2009, 9

[28] EUKARYOTIC PROTEINS EXPRESSED IN ESCHERICHIA-COLI - AN IMPROVED THROMBIN CLEAVAGE AND PURIFICATION PROCEDURE OF FUSION PROTEINS WITH GLUTATHIONE-S-TRANSFERASE [J].

GUAN, KL ;

DIXON, JE .

ANALYTICAL BIOCHEMISTRY, 1991, 192 (02) :262-267

[29] Identification and regulation of TPS04/GES, an Arabidopsis geranyllinalool synthase catalyzing the first step in the formation of the insect-induced volatile C16-homoterpene TMTT [J].

Herde, Marco ;

Gaertner, Katrin ;

Koellner, Tobias G. ;

Fode, Benjamin ;

Boland, Wilhelm ;

Gershenzon, Jonathan ;

Gatz, Christiane ;

Tholl, Dorothea .

PLANT CELL, 2008, 20 (04) :1152-1168

[30] Isolation of cDNAs and functional characterisation of two multi-product terpene synthase enzymes from sandalwood, Santalum album L. [J].

Jones, Christopher G. ;

Keeling, Christopher I. ;

Ghisalberti, Emilio L. ;

Barbour, Elizabeth L. ;

Plummer, Julie A. ;

Bohlmann, Joerg .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2008, 477 (01) :121-130

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