TTX-sensitive Na+ and nifedipine-sensitive Ca2+ channels in rat vas deferens smooth muscle cells

被引:15
作者
Belevych, AE
Zima, AV
Vladimirova, IA
Hirata, H
Jurkiewicz, A
Jurkiewicz, NH
Shuba, MF
机构
[1] Natl Acad Sci Ukraine, Bogomoletz Inst Physiol, Nerve Muscle Physiol Dept, UA-24 Kiev, Ukraine
[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Pharmacol, Sao Paulo, Brazil
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1999年 / 1419卷 / 02期
基金
巴西圣保罗研究基金会;
关键词
TTX-sensitive Na+ channel; L-type Ca2+ channel; vas deferens; smooth muscle; regulation; phosphorylation; patch-clamp;
D O I
10.1016/S0005-2736(99)00075-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The inward currents in single smooth muscle cells (SMC) isolated from epididymal part of rat vas deferens have been studied using whole-cell patch-clamp method. Depolarising steps from holding potential -90 mV evoked inward current with fast and slow components. The component with slow activation possessed voltage-dependent and pharmacological properties characteristic for Ca2+ current carried through L-type calcium channels (I-Ca). The fast component of inward current was activated at around -40 mV, reached its peak at 0 mV, and disappeared upon removal of Na ions from bath solution. This current was blocked in dose-dependent manner by tetrodotoxin (TTX) with an apparent dissociation constant of 6.7 nM. On the basis of voltage-dependent characteristics, TTX sensitivity of fast component of inward current and its disappearance in Na-free solution it is suggested that this current is TTX-sensitive depolarisation activated sodium current (I-Na) Cell dialysis with a pipette solution containing no macroergic compounds resulted in significant inhibition of I-Ca (depression of peak I-Ca by about 81% was observed by 13 min of dialysis), while I-Na remained unaffected during 50 min of dialysis. These data draw first evidence for the existence of TTX-sensitive Na+ current in single SMC isolated from rat vas deferens. These Na+ channels do not appear to be regulated by a phosphorylation process under resting conditions. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:343 / 352
页数:10
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