The effect of acteoside on histamine release and arachidonic acid release in RBL-2H3 mast cells

被引：58

作者：

Lee, Jin Hee ^{[1
]}

Lee, Ji Yun ^{[1
]}

Kang, Hyo Sulk ^{[1
]}

Jeong, Chan Hun ^{[1
]}

Moon, Hee ^{[1
]}

Whang, Wan Kyunn ^{[1
]}

Kim, Chang Jong ^{[1
]}

Sim, Sang Soo ^{[1
]}

机构：

[1] Chung Ang Univ, Coll Pharm, Dept Pathophysiol, Seoul 156756, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2006年 / 29卷 / 06期

关键词：

acteoside; histamine; arachidonic acid; prostaglandin E-2;

D O I：

10.1007/BF02969425

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The effect of acteoside, a phenylpropanoid glycoside isolated from Clerodendron-trichotomum Thunberg, on histamine and arachidonic acid release was investigated in RBL 2H3 cells. Histamine was dose-dependently released from RBL 2H3 cells by melittin, arachidonic acid and thapsigargin. In extracellular Ca2+-free solution, basal secretion of histamine increased by two fold. The response of histamine release to melittin and thapsigargin in Ca2+-free solution was significantly decreased, whereas the response to arachidonic acid was significantly increased as compared with those in normal solution. Acteoside inhibited histamine release induced by melittin, arachidonic acid and thapsigargin in a dose-dependent manner in the presence or absence of extracellular Ca2+. However, the inhibitory activity of acteoside was more potent in normal solution than that in Ca2+-free solution. These data suggest that inhibitory mechanism of acteoside on histamine release may be related to extracellular Ca2+. On the other hand, acteoside significantly inhibited arachidonic acid release and prostaglandin E-2 production induced by 0.5 mu M melittin. It is possible that acteoside may be developed as an anti-inflammatory agent.

引用

页码：508 / 513

页数：6

共 19 条

[1] Differential anti-inflammatory effects of immunosuppressive drugs:: Cyclosporin, rapamycin and FK-506 on inducible nitric oxide synthase, nitric oxide, cyclooxygenase-2 and PGE2 production [J].