Plant UVR8 Photoreceptor Senses UV-B by Tryptophan-Mediated Disruption of Cross-Dimer Salt Bridges

被引：360

作者：

Christie, John M. ^{[1
,2
,3
]}

Arvai, Andrew S. ^{[2
,3
]}

Baxter, Katherine J. ^{[1
]}

Heilmann, Monika ^{[1
]}

Pratt, Ashley J. ^{[2
,3
]}

O'Hara, Andrew ^{[1
]}

Kelly, Sharon M. ^{[1
]}

Hothorn, Michael ^{[4
]}

Smith, Brian O. ^{[1
]}

Hitomi, Kenichi ^{[2
,3
,5
,6
]}

Jenkins, Gareth I. ^{[1
]}

Getzoff, Elizabeth D. ^{[2
,3
]}

机构：

[1] Univ Glasgow, Inst Mol Cell & Syst Biol, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland

[2] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[4] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA

[5] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Life Sci Div, Berkeley, CA 94720 USA

[6] Natl Inst Biomed Innovat, Sect Lab Equipment, Osaka 5670085, Japan

来源：

SCIENCE | 2012年 / 335卷 / 6075期

基金：

美国国家科学基金会; 英国生物技术与生命科学研究理事会; 美国国家卫生研究院;

关键词：

SIGNALING COMPONENT UVR8; SCATTERING SAXS; ARABIDOPSIS; TRANSDUCTION; ASSEMBLIES; CHROMATIN; PROTEINS; TISSUE;

D O I：

10.1126/science.1218091

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The recently identified plant photoreceptor UVR8 (UV RESISTANCE LOCUS 8) triggers regulatory changes in gene expression in response to ultraviolet-B (UV-B) light through an unknown mechanism. Here, crystallographic and solution structures of the UVR8 homodimer, together with mutagenesis and far-UV circular dichroism spectroscopy, reveal its mechanisms for UV-B perception and signal transduction. beta-propeller subunits form a remarkable, tryptophan-dominated, dimer interface stitched together by a complex salt-bridge network. Salt-bridging arginines flank the excitonically coupled cross-dimer tryptophan "pyramid" responsible for UV-B sensing. Photoreception reversibly disrupts salt bridges, triggering dimer dissociation and signal initiation. Mutation of a single tryptophan to phenylalanine retunes the photoreceptor to detect UV-C wavelengths. Our analyses establish how UVR8 functions as a photoreceptor without a prosthetic chromophore to promote plant development and survival in sunlight.

引用

页码：1492 / 1496

页数：5

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