B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells

被引：62

作者：

Mansson, Robert ^{[1
,2
,3
]}

Zandi, Sasan ^{[1
]}

Anderson, Kristina ^{[2
,3
]}

Martensson, Inga-Lill ^{[4
]}

Jacobsen, Sten Eirik W. ^{[2
,3
]}

Bryder, David ^{[5
]}

Sigvardsson, Mikael ^{[1
,2
,3
]}

机构：

[1] Linkoping Univ, Dept Biomed & Surg, Linkoping, Sweden

[2] Lund Univ, Hematopoiet Stem Cell Lab, Lund, Sweden

[3] Lund Univ, Lund Strateg Res Ctr Stem Cell Biol & Cell Therap, Lund, Sweden

[4] Babraham Inst, Lab Lymphocyte Signaling & Dev, Cambridge, England

[5] Lund Univ, Dept Immunol, Lund, Sweden

来源：

BLOOD | 2008年 / 112卷 / 04期

关键词：

D O I：

10.1182/blood-2007-11-125385

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda 5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220(-)CD19(-)CD127(+)FLT3(+) SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19.

引用

页码：1048 / 1055

页数：8

共 38 条

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