RNA-Seq: a revolutionary tool for transcriptomics

被引：8789

作者：

Wang, Zhong ^{[1
]}

Gerstein, Mark ^{[2
]}

Snyder, Michael ^{[1
]}

机构：

[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA

[2] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

来源：

NATURE REVIEWS GENETICS | 2009年 / 10卷 / 01期

关键词：

LARGE-SCALE ANALYSIS; GENE-EXPRESSION; YEAST GENOME; CAP ANALYSIS; DISCOVERY; IDENTIFICATION; MAPS;

D O I：

10.1038/nrg2484

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.

引用

页码：57 / 63

页数：7

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