Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution

被引:877
作者
Cheng, J
Kapranov, P
Drenkow, J
Dike, S
Brubaker, S
Patel, S
Long, J
Stern, D
Tammana, H
Helt, G
Sementchenko, V
Piccolboni, A
Bekiranov, S
Bailey, DK
Ganesh, M
Ghosh, S
Bell, I
Gerhard, DS
Gingeras, TR [1 ]
机构
[1] Affymetrix Inc, Santa Clara, CA 95051 USA
[2] NCI, Off Canc Genom, Bethesda, MD 20892 USA
关键词
D O I
10.1126/science.1108625
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Sites of transcription of polyadenylated and nonpolyadenylated RNAs for 10 human chromosomes were mapped at 5-base pair resolution in eight cell lines. Unannotated, nonpotyadenylated transcripts comprise the major proportion of the transcriptional output of the human genome. Of all transcribed sequences, 19.4, 43.7, and 36.9% were observed to be polyadenytated, nonpolyadenylated, and bimorphic, respectively. Half of all transcribed sequences are found only in the nucleus and for the most part are unannotated. Overall, the transcribed portions of the human genome are predominantly composed of interlaced networks of both poly A+ and poly A- annotated transcripts and unannotated transcripts of unknown function. This organization has important implications for interpreting genotype-phenotype associations, regulation of gene expression, and the definition of a gene.
引用
收藏
页码:1149 / 1154
页数:6
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