Sensitive and robust detection of citrus greening (huanglongbing) bacterium "Candidatus Liberibacter asiaticus" by DNA amplification with new 16S rDNA-specific primers

被引：57

作者：

Fujikawa, Takashi ^{[1
]}

Iwanami, Toru ^{[1
]}

机构：

[1] Natl Inst Fruit Tree Sci NIFTS, Tsukuba, Ibaraki 3058605, Japan

来源：

MOLECULAR AND CELLULAR PROBES | 2012年 / 26卷 / 05期

关键词：

Citrus greening; Huanglongbing; Liberibacter asiaticus; DNA amplification; 16S rDNA; Diagnosis; SAO-PAULO STATE; MULTIPLE SEQUENCE ALIGNMENT; PCR DETECTION; DISEASE; AMERICANUS; AFRICANUS; BRAZIL; TREE;

D O I：

10.1016/j.mcp.2012.06.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Citrus greening disease is caused by "Candidatus Liberibacter spp.,"including "Candidatus Liberibacter asiaticus (Las)." For detecting this disease, we designed new primers from the Las 16S rDNA and used a very small DNA template for PCR. More Las-infected tissues were detected with our primers than with the common primers. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：194 / 197

页数：4

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