Complete Genome Sequence of Citrus Huanglongbing Bacterium, 'Candidatus Liberibacter asiaticus' Obtained Through Metagenomics

被引：435

作者：

Duan, Yongping ^{[1
]}

Zhou, Lijuan ^{[2
]}

Hall, David G. ^{[1
]}

Li, Wenbin ^{[3
]}

Doddapaneni, Harshavardhan ^{[4
]}

Lin, Hong ^{[4
]}

Liu, Li ^{[5
]}

Vahling, Cheryl M. ^{[1
]}

Gabriel, Dean W. ^{[2
]}

Williams, Kelly P. ^{[6
]}

Dickerman, Allan ^{[6
]}

Sun, Yijun ^{[5
]}

Gottwald, Tim ^{[1
]}

机构：

[1] USDA ARS, USHRL, Ft Pierce, FL 34945 USA

[2] Univ Florida, Dept Plant Pathol, Gainesville, FL 32653 USA

[3] USDA, APHIS, PPQ, CPHST, Beltsville, MD 20705 USA

[4] USDA ARS, Parlier, CA 93648 USA

[5] Univ Florida, ICBR, Gainesville, FL 32653 USA

[6] Virginia Tech, Virginia Bioinformat Inst, Blacksburg, VA 24061 USA

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2009年 / 22卷 / 08期

关键词：

XYLELLA-FASTIDIOSA; ESCHERICHIA-COLI; GREENING DISEASE; GENE; SECRETION; IDENTIFICATION; PATHOGENICITY; WOLBACHIA; TRANSPORT; EVOLUTION;

D O I：

10.1094/MPMI-22-8-1011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Citrus huanglongbing is the most destructive disease of citrus worldwide. It is spread by citrus psyllids and is associated with a low-titer, phloem-limited infection by any of three uncultured species of alpha-Proteobacteria, 'Candidatus Liberibacter asiaticus', 'Ca. L. americanus', and 'Ca. L. africanus'. A complete circular 'Ca. L. asiaticus' genome has been obtained by metagenomics, using the DNA extracted from a single 'Ca. L. asiaticus'-infected psyllid. The 1.23-Mb genome has an average 36.5% GC content. Annotation revealed a high percentage of genes involved in both cell motility (4.5%) and active transport in general (8.0%), which may contribute to its virulence. 'Ca. L. asiaticus' appears to have a limited ability for aerobic respiration and is likely auxotrophic for at least five amino acids. Consistent with its intracellular nature, 'Ca. L. asiaticus' lacks type III and type IV secretion systems as well as typical free-living or plant-colonizing extracellular degradative enzymes. 'Ca. L. asiaticus' appears to have all type I secretion system genes needed for both multidrug efflux and toxin effector secretion. Multiprotein phylogenetic analysis confirmed 'Ca. L. asiaticus' as an early-branching and highly divergent member of the family Rhizobiaceae. This is the first genome sequence of an uncultured alpha-proteobacteria that is both an intracellular plant pathogen and insect symbiont.

引用

页码：1011 / 1020

页数：10

共 74 条

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