MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins

被引：67

作者：

Davies, Stefan M. K. ^{[1
,2
]}

Sanchez, Maria I. G. Lopez ^{[1
,2
]}

Narsai, Reena ^{[3
]}

Shearwood, Anne-Marie J. ^{[1
,2
]}

Razif, Muhammad F. M. ^{[1
,2
]}

Small, Ian D. ^{[3
]}

Whelan, James ^{[3
]}

Rackham, Oliver ^{[1
,2
]}

Filipovska, Aleksandra ^{[1
,2
]}

机构：

[1] Univ Western Australia, Western Australian Inst Med Res, Perth, WA 6000, Australia

[2] Univ Western Australia, Med Res Ctr, Perth, WA 6000, Australia

[3] Univ Western Australia, Australian Res Council, Ctr Excellence Plant Energy Biol, Perth, WA 6000, Australia

来源：

FEBS LETTERS | 2012年 / 586卷 / 20期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

Pentatricopeptide repeat domain; RNA-binding protein; RNA; IN-VIVO; TRANSFER-RNA; GENE; EXPRESSION; TRANSCRIPTION; RIBOSOME; LRPPRC; DNA; IDENTIFICATION; MUTATION;

D O I：

10.1016/j.febslet.2012.07.043

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNA(Glu), however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity.

引用

页码：3555 / 3561

页数：7

共 41 条

[1]

Elongation in translation as a dynamic interaction among the ribosome, tRNA, and elongation factors EF-G and EF-Tu [J].