Pentatricopeptide repeat domain protein 3 associates with the mitochondrial small ribosomal subunit and regulates translation

被引:89
作者
Davies, Stefan M. K. [1 ]
Rackham, Oliver [1 ]
Shearwood, Anne-Marie J. [1 ]
Hamilton, Kristina L. [1 ]
Narsai, Reena [2 ]
Whelan, James [2 ]
Filipovska, Aleksandra [1 ]
机构
[1] Univ Western Australia, Med Res Ctr, Western Australian Inst Med Res, Perth, WA 6000, Australia
[2] Univ Western Australia, Australian Res Council, Ctr Excellence Plant Energy Biol, Nedlands, WA 6009, Australia
来源
FEBS LETTERS | 2009年 / 583卷 / 12期
基金
英国医学研究理事会;
关键词
Pentatricopeptide repeat domain; RNA-binding protein; rRNA; Mitochondrial gene expression; OXIDATIVE-PHOSPHORYLATION; MAMMALIAN MITOCHONDRIA; GENE-EXPRESSION; MESSENGER-RNA; NUCLEAR; CELLS; IDENTIFICATION; STABILITY;
D O I
10.1016/j.febslet.2009.04.048
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The basic components and mechanisms of mitochondrial transcription in mammals have been described, however, the components involved in mRNA processing, translation and stability remain largely unknown. In plants, pentatricopeptide domain RNA-binding proteins regulate the stability, expression and translation of mitochondrial transcripts. Here, we investigated the role of an uncharacterized mammalian pentatricopeptide domain protein, pentatricopeptide repeat domain protein 3 (PTCD3), and showed that it is a mitochondrial protein that associates with the small subunit of mitochondrial ribosomes. PTCD3 knockdown and over expression did not affect mitochondrial mRNA levels, suggesting that PTCD3 is not involved in RNA processing and stability. However, lowering PTCD3 in 143B osteosarcoma cells decreased mitochondrial protein synthesis, mitochondrial respiration and the activity of Complexes III and IV, suggesting that PTCD3 has a role in mitochondrial translation. Structured summary: MINT-7033995: PTCD3 (uniprotkb: Q96EY7) associates (MI: 0914) with MRPS15 (uniprotkb: P82914) by tandem affinity purification (MI: 0676) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:1853 / 1858
页数:6
相关论文
共 25 条
[1]   A large-scale screening of the normalized mammalian mitochondrial gene expression profiles [J].
Anisimov, SV .
GENETICAL RESEARCH, 2005, 86 (02) :127-138
[2]   An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells [J].
Buerckstuemmer, Tilmann ;
Bennett, Keiryn L. ;
Preradovic, Adrijana ;
Schutze, Gregor ;
Hantschel, Oliver ;
Superti-Furga, Giulio ;
Bauch, Angela .
NATURE METHODS, 2006, 3 (12) :1013-1019
[3]   Tissue-specific stability of nuclear- and mitochondrially encoded mRNAs [J].
Connor, MK ;
Takahashi, M ;
Hood, DA .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1996, 333 (01) :103-108
[4]   DNA replication and transcription in mammalian mitochondria [J].
Falkenberg, Maria ;
Larsson, Nils-Goeran ;
Gustafsson, Claes M. .
ANNUAL REVIEW OF BIOCHEMISTRY, 2007, 76 :679-699
[5]   Translational control of endogenous and recoded nuclear genes in yeast mitochondria: Regulation and membrane targeting [J].
Fox, TD .
EXPERIENTIA, 1996, 52 (12) :1130-1135
[6]   Messenger RNA stability in mitochondria: different means to an end [J].
Gagliardi, D ;
Stepien, PP ;
Temperley, RJ ;
Lightowlers, RN ;
Chrzanowska-Lightowlers, ZMA .
TRENDS IN GENETICS, 2004, 20 (06) :260-267
[7]   Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes [J].
Gur, Y ;
Breitbart, H .
GENES & DEVELOPMENT, 2006, 20 (04) :411-416
[8]   The effect of mutated mitochondrial ribosomal proteins S16 and S22 on the assembly of the small and large ribosomal subunits in human mitochondria [J].
Haque, Md. Emdadul ;
Grasso, Domenick ;
Miller, Chaya ;
Spremulli, Linda L. ;
Saada, Ann .
MITOCHONDRION, 2008, 8 (03) :254-261
[9]   RNase P without RNA: Identification and Functional Reconstitution of the Human Mitochondrial tRNA Processing Enzyme [J].
Holzmann, Johann ;
Frank, Peter ;
Loeffler, Esther ;
Bennett, Keiryn L. ;
Gerner, Christopher ;
Rossmanith, Walter .
CELL, 2008, 135 (03) :462-474
[10]   Altered mitochondrial function in fibroblasts containing MELAS or MERRF mitochondrial DNA mutations [J].
James, AM ;
Wei, YH ;
Pang, CY ;
Murphy, MP .
BIOCHEMICAL JOURNAL, 1996, 318 :401-407