Differential involvement of PKA and PKC in regulation of catecholamine enzyme genes by PACAP

被引：30

作者：

Choi, HJ ^{[1
]}

Park, SY ^{[1
]}

Hwang, O ^{[1
]}

机构：

[1] Univ Ulsan, Coll Med, Dept Biochem, Seoul 138736, South Korea

来源：

PEPTIDES | 1999年 / 20卷 / 07期

关键词：

protein kinase A; tyrosine hydroxylase; dopamine beta-hydroxylase; phenylethanolamine N-methyltransferase; pituitary adenylate cyclase-activating polypeptide; protein kinase C;

D O I：

10.1016/S0196-9781(99)00067-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Roles of protein kinase A (PKA) and protein kinase C (PKC) in regulation of tyrosine hydroxylase, dopamine beta-hydroxylase, and phenylethanolamine N-methyltransferase expression by pituitary adenylate cyclase-activating polypeptide (PACAP) were determined in primary cultured bovine chromaffin cells. DBH up-regulation by PACAP was reduced by H-89 and not further increased by forskolin showing involvement of cAMP/PKA. It was not mediated by PKC, as 12-O-tetradecanoylphorbol-13-acetate and sphingosine exerted no effect. Tyrosine hydroxylase induction by PACAP was mediated by both kinases. The PACAP-activated PKA up-regulated phenylethanolamine N-methyltransferase expression whereas PKC caused down-regulation. PACAP increased tyrosine hydroxylase and dopamine beta-hydroxylase activities, but slightly lowered phenylethanolamine N-methyltransferase activity, resulting in a preferential rise in norepinephrine over epinephrine. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：817 / 822

页数：6

共 34 条

[21]

Park SY, 1999, MOL CELLS, V9, P146

[22] Cloning and characterization of the signal transduction of four splice variants of the human pituitary adenylate cyclase activating polypeptide receptor - Evidence for dual coupling to adenylate cyclase and phospholipase C [J].

Pisegna, JR ;

Wank, SA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (29) :17267-17274

[23] PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) POTENTLY ENHANCES TYROSINE-HYDROXYLASE (TH) EXPRESSION IN ADRENAL CHROMAFFIN CELLS [J].

RIUS, RA ;

GUIDOTTI, A ;

COSTA, E .

LIFE SCIENCES, 1994, 54 (22) :1735-1743

[24]

ROSS ME, 1990, J NEUROSCI, V10, P520

[25] Effects of pituitary adenylate cyclase-activating polypeptide on cardiovascular and respiratory responses in anaesthetised dogs [J].

Runcie, MJ ;

Ulman, LG ;

Potter, EK .

REGULATORY PEPTIDES, 1995, 60 (2-3) :193-200

[26]

SABBAN EL, 1983, J BIOL CHEM, V258, P7819

[27] STRUCTURE OF HUMAN PHENYLETHANOLAMINE N-METHYLTRANSFERASE GENE - EXISTENCE OF 2 TYPES OF MESSENGER-RNA WITH DIFFERENT TRANSCRIPTION INITIATION SITES [J].

SASAOKA, T ;

KANEDA, N ;

KUROSAWA, Y ;

FUJITA, K ;

NAGATSU, T .

NEUROCHEMISTRY INTERNATIONAL, 1989, 15 (04) :555-565

[28]

SHASKUS J, 1992, J BIOL CHEM, V267, P18821

[29] COORDINATE AND DIFFERENTIAL REGULATION OF PHENYLETHANOLAMINE N-METHYLTRANSFERASE, TYROSINE-HYDROXYLASE AND PROENKEPHALIN MESSENGER-RNAS BY NEURAL AND HORMONAL MECHANISMS IN CULTURED BOVINE ADRENAL-MEDULLARY CELLS [J].

STACHOWIAK, MK ;

HONG, JS ;

VIVEROS, OH .

BRAIN RESEARCH, 1990, 510 (02) :277-288

[30] Pituitary adenylate cyclase activating polypeptide (PACAP) regulates expression of catecholamine biosynthetic enzyme genes in bovine adrenal chromaffin cells [J].

Tonshoff, C ;

Hemmick, L ;

Evinger, MJ .

JOURNAL OF MOLECULAR NEUROSCIENCE, 1997, 9 (02) :127-140

← 1 2 3 4 →