Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P-falciparum

被引：60

作者：

Lobo, CA

Fujioka, H

Aikawa, M

Kumar, N ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21205 USA

[2] Case Western Reserve Univ, Sch Med, Inst Pathol, Cleveland, OH 44106 USA

[3] Tokai Univ, Inst Med Sci, Kanagawa 2591193, Japan

来源：

MOLECULAR CELL | 1999年 / 3卷 / 06期

关键词：

D O I：

10.1016/S1097-2765(01)80011-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transmission of malaria depends upon the differentiation and development of the sexual stages of the parasite. In Plasmodium falciparum, it is a complex, multistage process, involving the expression of a large number of sexual stage-specific proteins. Pfg27 is one such protein, abundantly expressed at the onset of gametocytogenesis, We report successful disruption of the Pfg27 locus using homologous recombination and show that it is essential for the maintenance of the sexual phenotype. Transfectants lacking Pfg27 abort early in sexual development, resulting in vacuolated, highly disarranged, and disintegrating parasites. This suggests a critical role for Pfg27 in the sexual development of the parasite.

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页码：793 / 798

页数：6

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